Human SIRP delta Antibody

Catalog #: MAB10138 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB10138-100
MAB10138-SP

Detection of SIRP delta on HEK293 Human Cell Line transfected with Human SIRP delta by Flow Cytometry.

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All SIRP delta Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SIRP delta Antibody Summary

Species Reactivity

Human

Specificity

Detects human SIRP delta in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2588A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell, HEK293-derived human SIRP delta
Phe30-Arg197
Accession # Q9H106

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

3-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SIRP delta on HEK293 Human Cell Line transfected with Human SIRP delta by Flow Cytometry. HEK293 cells transfected with Human SIRP delta (filled histogam) or irrelevant protein (open histogram) were stained with Rabbit Anti-Human SIRP delta Monoclonal Antibody (Catalog # MAB10138) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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SIRP delta in THP‑1 Human Cell Line. SIRP delta was detected in immersion fixed THP-1 human acute monocytic leukemia cell line (left panel; positive staining) and MCF-7 human breast cancer cell line (right panel; negative staining) using Rabbit Anti-Human SIRP delta Monoclonal Antibody (Catalog # MAB10138) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP delta

SIRPD (Signal Regulatory Protein Delta), also know as Protein Tyrosine Phosphatase non-Receptor Type Substrate 1-l Like 2 (PTPNS1L2), is member of the signal regulatory proteins (SIRPS) family (1). SIRPD contains a 168 amino acid Ig-like domain that is characteristic of other SIRP family members (1). Unlike other members of SIRPS family, SIRPD lacks the transmemberane region, and is secreted (2). Murine homologs of SIRPD are not characterized. Expression sequence tag analysis suggests that SIRPD may be expressed in sperm cells and respiratory tissue (2). Using BioPlex 2.0 (Biophysical Interactions of ORFeome-derived complexes) high-throughput affinity purification–mass spectrometry (AP–MS) analysis to identify probable protein–protein interactions, several candidate SIRPD interactions were found including DIRAS2 (3). In-house testing indicates SIRPD can interact with DIRAS2.

References

Van den Berg, T.K. et al. (2005) J. Immunol. 175:7788.
Van Beek, E.M. et al. (2005) J Immunol. 175:7781.
Huttlin, E.L. et al. (2017) Nature 545:505.

Long Name

Signal-regulatory Protein delta

Entrez Gene IDs

128646 (Human)

Alternate Names

dJ576H24.4; Protein tyrosine phosphatase non-receptor type substrate 1-like 2; protein tyrosine phosphatase, non-receptor type substrate 1-like 2; PTPNS1L2; signal-regulatory protein delta; SIRP delta; SIRPD; SIRP-Delta