Human SIRP alpha /CD172a
Antibody

Catalog #: MAB45462 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB45462-100
MAB45462-SP

Human SIRP alpha /CD172a ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SIRP alpha /CD172a
Antibody Summary

Species Reactivity

Human

Specificity

Detects human SIRP alpha /CD172a in ELISA.

Source

Monoclonal Mouse IgG₁ Clone # 602407

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha /CD172a
Gly27-Asn370
Accession # P78324

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB45461).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human SIRP alpha DuoSet ELISA (Catalog # DY4546-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human SIRP alpha /CD172a ELISA Standard Curve. Recombinant Human SIRP alpha /CD172a protein was serially diluted 2-fold and captured by Mouse Anti-Human SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB45462) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SIRP alpha /CD172a Monoclonal Antibody (Catalog # MAB45461) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP alpha/CD172a

SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.

Long Name

Signal-regulatory Protein alpha

Entrez Gene IDs

140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)

Alternate Names

BIT; BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs; Brain Ig-like molecule with tyrosine-based activation motifs; CD172 antigen-like family member A; CD172a antigen; CD172a; Inhibitory receptor SHPS-1; Macrophage fusion receptor; MFR; MFRtyrosine phosphatase SHP substrate 1; MyD-1 antigen; MYD1; MYD-1; P84; protein tyrosine phosphatase, non-receptor type substrate 1; PTPNS1; SHP substrate 1; SHPS1; SHPS-1; SHPS1CD172A; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; SIRP alpha; SIRPA; SIRPalpha; Sirp-alpha-1; SIRPalpha2; Sirp-alpha-2; Sirp-alpha-3; SIRPtyrosine-protein phosphatase non-receptor type substrate 1