Human SIRP gamma /CD172g Antibody

Catalog #: MAB4486 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4486
MAB4486-SP

Detection of Human SIRP gamma /CD172g by Western Blot.

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Human SIRP gamma /CD172g Antibody Summary

Species Reactivity

Human

Specificity

Detects human SIRP gamma /CD172g in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant human (rh) SIRP alpha and rhSIRP beta 1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 595337

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SIRP gamma /CD172g
Val64-Ser364
Accession # Q9P1W8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SIRP gamma /CD172g by Western Blot. Western blot shows lysates of NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP gamma/CD172g Monoclonal Antibody (Catalog # MAB4486) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SIRP gamma/CD172g at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP gamma/CD172g

Signal-regulatory protein gamma/beta 2 (SIRP gamma/beta 2) is a 45-47 kDa type 1 transmembrane glycoprotein and member of the immunoglobulin superfamily and SIRP family of proteins. Human SIRP gamma/beta 2 is synthesized as a 387 amino acid (aa) precursor that contains a 28 aa signal sequence, a 332 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 4 aa cytoplasmic tail. The ECD contains two Ig-like C1-type domains, one Ig-like V-type domain, and four potential sites for N-linked glycosylation. There are four named isoforms for human SIRP gamma/beta 2. Human SIRP gamma/beta 2 has no orthologs, but it is 75% aa identical to SIRP beta. It is expressed in the liver and at very low levels in brain, heart, lung, pancreas, kidney, placenta, and skeletal muscle. SIRP gamma/beta 2 is also expressed on CD4⁺ T-cells, CD8⁺ T-cells, and activated NK cells.

Long Name

Signal-regulatory Protein gamma

Entrez Gene IDs

55423 (Human); 102138723 (Cynomolgus Monkey)

Alternate Names

bA77C3.1; CD172g antigen; CD172g; FLJ42230; signal-regulatory protein beta 2; Signal-regulatory protein beta-2; signal-regulatory protein gamma; SIRP beta 2; SIRP gamma; SIRP-B2; SIRPB2CD172 antigen-like family member B; SIRP-beta-2; SIRPG; SIRPgamma; SIRP-gamma