Human Sirtuin 2/SIRT2 Antibody

Catalog #: MAB4358
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4358
MAB4358-SP

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Sirtuin 2/SIRT2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sirtuin 2/SIRT2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SIRT1 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 506611

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 2/SIRT2
Ala2-Gln389
Accession # Q8IXJ6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)

Immunoprecipitation

25 µg/mL

Cell lysates spiked with Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 2/SIRT2

Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1, 2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3, 4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).

References

Frye, R.A. (1999) Biochem. Biophys. Res. Commun. 260:273.
North, B.J. et al. (2003) Mol. Cell. 11:437.
North, B.J. and Verdin, E. (2004) Genome Biol. 5:224.
Marmorstein, R. (2004) Biochem. Soc. Trans. 32:904.
Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262:75.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Entrez Gene IDs

22933 (Human); 64383 (Mouse); 361532 (Rat)

Alternate Names

EC 3.5.1; EC 3.5.1.-; FLJ35621; FLJ37491; S.cerevisiae, homolog) 2; SIR2L2; SIRT2; Sirtuin 2; sirtuin type 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Sirtuin 2/SIRT2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Influenza Virus Down-Modulates G6PD Expression and Activity to Induce Oxidative Stress and Promote Its Replication
Authors: Marta De Angelis, Donatella Amatore, Paola Checconi, Alessandra Zevini, Alessandra Fraternale, Mauro Magnani et al.
Frontiers in Cellular and Infection Microbiology