Human Sirtuin 2/SIRT2 Antibody

Catalog #: AF4358 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4358
AF4358-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Sirtuin 2/SIRT2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Sirtuin 2/SIRT2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human SIRT1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 2/SIRT2
Ala2-Gln389 (predicted)
Accession # Q8IXJ6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 2/SIRT2

Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1‑2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3‑4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).

References