Human SLC14A1 Antibody

Catalog #: MAB8238 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8238
MAB8238-SP

Detection of SLC14A1 in Human Red Blood Cells by Flow Cytometry.

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All SLC14A1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SLC14A1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLC14A1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 888418

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC14A1
Accession # Q13336

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SLC14A1 in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Flow Cytometry

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Detection of SLC14A1 in HEK293 Human Cell Line Transfected with Human SLC14A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP was stained with either (A) Mouse Anti-Human SLC14A1 Monoclonal Antibody (Catalog # MAB8238) or (B) Mouse IgG_2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC14A1

Urea transporter 1, designated Solute Carrier family 14 member 1 (SLC14A1) is a 43-54 kDa multipass urea transporter mainly expressed in erythrocytes, but also found in kidney, mesenchymal stem cells, and some carcinomas. It contains an epitope identified as the Kidd (JK) blood group antigen. The antigenic forms differ at only one amino acid (aa); Asp280 represents Jk(a) and Asn280 represents Jk(b). SLC14A1 Isoform 2 (445 aa) contains an extended N-terminus as compared to Isoform 1 (389 aa). Human SLC14A1 shares 83% and 81% aa sequence identity with mouse and rat SLC14A1, respectively.

Long Name

Solute Carrier Family 14 (Urea Transporter), Member 1 (Kidd Blood Group

Entrez Gene IDs

6563 (Human); 108052 (Mouse); 54301 (Rat)

Alternate Names

FLJ33745; HsT1341; HUT11; JK; JKFLJ41687; RACH1; RACH2; SLC14A1; solute carrier family 14 (urea transporter), member 1 (Kidd blood group); Solute carrier family 14 member 1; truncated urea transporter; urea transporter 1; urea transporter JK glycoprotein; Urea transporter, erythrocyte; urea transporter-B1; UT1; UT1UT-B1; UT-B1; UTE; UTEblood group Kidd urea transporter