Human SLC14A1 APC-conjugated Antibody

Name: Human SLC14A1 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB8238A

Catalog #: FAB8238A Datasheet / COA / SDS

Discontinued Product

FAB8238A has been discontinued.

View all SLC14A1 products.

Detection of SLC14A1 in Human Red Blood Cells by Flow Cytometry.

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Human SLC14A1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human SLC14A1 in ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 888418

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human SLC14A1
Accession # Q13336

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of SLC14A1 in Human Red Blood Cells by Flow Cytometry. Human red blood cells were stained with Mouse Anti-Human SLC14A1 APC-conjugated Mono-clonal Antibody (Catalog # FAB8238A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of SLC14A1 in HEK293 Human Cell Line Transfected with Human SLC14A1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human SLC14A1 and eGFP was stained with either (A) Mouse Anti-Human SLC14A1 APC-conjugated Monoclonal Antibody (Catalog # FAB8238A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: SLC14A1

Urea transporter 1, designated Solute Carrier family 14 member 1 (SLC14A1) is a 43-54 kDa multipass urea transporter mainly expressed in erythrocytes, but also found in the kidney, mesenchymal stem cells, and some carcinomas. It contains an epitope identified as the Kidd (JK) blood group antigen. The antigenic forms differ at only one amino acid (aa); Asp280 represents Jk(a) and Asn280 represents Jk(b). SLC14A1 Isoform 2 (445 aa) contains an extended N-terminus as compared to Isoform 1 (389 aa). Human SLC14A1 shares 83% and 81% aa sequence identity with mouse and rat SLC14A1, respectively.

Long Name

Solute Carrier Family 14 (Urea Transporter), Member 1 (Kidd Blood Group

Entrez Gene IDs

6563 (Human); 108052 (Mouse); 54301 (Rat)

Alternate Names

FLJ33745; HsT1341; HUT11; JK; JKFLJ41687; RACH1; RACH2; SLC14A1; solute carrier family 14 (urea transporter), member 1 (Kidd blood group); Solute carrier family 14 member 1; truncated urea transporter; urea transporter 1; urea transporter JK glycoprotein; Urea transporter, erythrocyte; urea transporter-B1; UT1; UT1UT-B1; UT-B1; UTE; UTEblood group Kidd urea transporter