Human SLC22A2/OCT2 Antibody Summary
Accession # O15244
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of SLC22A2/OCT2 in Human Kidney via seqIF™ staining on COMET™ SLC22A2?/OCT2 Antibody was detected in immersion fixed paraffin-embedded sections of human Kidney using Mouse Anti-Human SLC22A2/OCT2, Monoclonal Antibody (Catalog # MAB6547) at 20ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
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SLC22A2 in Human Kidney. SLC22A2 was detected in immersion fixed paraffin-embedded sections of human kidney using Human SLC22A2 Monoclonal Antibody (Catalog # MAB6547) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of epithelial cells in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of SLC22A2/OCT2 in HEK293 Human Cell Line Transfected with Human SLC22A2/OCT2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with EGP and (A) human SLC22A2/OCT2 or (B) irrelevant protein was stained with Mouse Anti-Human SLC22A2/OCT2 Monoclonal Antibody (Catalog # MAB6547) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on Mouse IgG2A Isotype Control (MAB003). Staining was performed using our Staining Membrane-associated Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SLC22A2/OCT2
Solute carrier family 22 member 2 (SLC22A2; also hOCT2) is a 65 kDa member of the major facilitator superfamily and organic cation transporter family of proteins. Human SLC22A2 is synthesized as a multipass transmembrane protein that is 555 amino acids (aa) in length. Human SLC22A2 contains one potential site for N-linked glycosylation. There are also two additional isoforms for human SLC22A2. Isoform 2 has a 57 aa substitution for aa 427-483 and a deletion of the 72 aa at positions 484-555. Isoform 3 has an 18 aa substitution for aa 225-242 and a deletion of residues 243-555. SLC22A2 has its highest expression in the kidney. It is also expressed at lower levels in neurons of the cerebral cortex and in various subcortical nuclei.
Product Datasheets
Citations for Human SLC22A2/OCT2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Generation of an induced pluripotent stem cell (iPSC) line (EXSURGi001-A) from a patient homozygous for the p.Ala165Thr mutation in the MTARC1 gene
Authors: Tang, P;Keshi, E;Wilken, S;Wutsdorff, L;Mougnekabol, J;Pratschke, J;Sauer, IM;Haep, N;
Stem cell research
Species: Human
Sample Types: Embryoid Bodies
Applications: Immunohistochemistry -
Comment on “Epigenetic activation of the drug transporter OCT2 sensitizes renal cell carcinoma to oxaliplatin”
Authors: Stefan Winter, Pascale Fisel, Florian Büttner, Anne T. Nies, Arnulf Stenzl, Jens Bedke et al.
Science Translational Medicine
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Response to Comment on “Epigenetic activation of the drug transporter OCT2 sensitizes renal cell carcinoma to oxaliplatin”
Authors: Xiaoli Zheng, Yanqing Liu, Qinqin Yu, Hua Wang, Fuqing Tan, Qianying Zhu et al.
Science Translational Medicine
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