Human Slug PE-conjugated Antibody

Catalog #: IC7408P Datasheet / COA / SDS

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IC7408P

Detection of Slug in HeLa Human Cell Line by Flow Cytometry.

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All Slug Antibodies

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Human Slug PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Slug in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 666633

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Slug
Met1-His268
Accession # O43623

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Slug antibody in HeLa Human Cell Line antibody by Flow Cytometry.

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Detection of Slug in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Slug PE-conjugated Monoclonal Antibody (Catalog # IC7408P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Slug

SLUG, also known as SNAI2, is a zinc-finger transcription factor. SLUG is a transcriptional repressor which may suppress E-cadherin transcription and be involved in an epithelial to mesenchymal transition (EMT).

Entrez Gene IDs

6591 (Human); 20583 (Mouse)

Alternate Names

MGC10182; Neural crest transcription factor Slug; Protein snail homolog 2; slug homolog, zinc finger protein (chicken); Slug; SLUGH; SLUGH1; SLUGzinc finger protein; SNAI2; snail 2; snail homolog 2 (Drosophila); SNAIL2; WS2D; zinc finger protein SNAI2