Human SMPD1 Antibody

Catalog #: AF5348
5 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5348
AF5348-SP

Detection of Human SMPD1 by Western Blot.

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All SMPD1 Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SMPD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SMPD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse SMPD1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SMPD1
His62-Pro628
Accession # NP_000534

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

Human brain (cerebellum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SMPD1 by Western Blot. Western blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SMPD1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western

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Detection of Human SMPD1 by Simple Western^TM. Simple Western lane view shows lysates of human brain (cerebellum), loaded at 0.2 mg/mL. A specific band was detected for SMPD1 at approximately 87 kDa (as indicated) using 50 µg/mL of Goat Anti-Human SMPD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5348). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SMPD1

Sphingomyelin phosphodiesterase, also known as acid sphingomyelinase and encoded by the SMPD1 gene, is a lysosomal phosphodiesterase which belongs to the acid sphingomyelinase family (1). SMPD1 catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. Ceramide, a bioactive lipid, has emerged as an important signaling molecule involved in a variety of cellular processes such as cell differentiation, apoptosis, and proliferation (2). Activation of SMPD1 occurs by the removal, chemical modification or dimerization of its C-terminal cysteine residue (3). Deficiencies of SMPD1 result in a lysosomal storage disorder referred to as Niemann-Pick disease (4). rhSMPD1 was expressed without the last three C-terminal residues, and is therefore constitutively active.

References

Schuchman, E.H. et al. (1991) J. Biol. Chem. 266:8531.
Melendez, A.J. et al. (2008) Biochim. Biophys. Acta 1784:66.
Qiu, H. et al. (2003) J. Biol. Chem. 278:32744.
Smith, E.L. and Schuchman, E.H. (2008) FASEB J. 22:3419.

Long Name

Sphingomyelin Phosphodiesterase 1, Acid Lysosomal

Entrez Gene IDs

6609 (Human); 20597 (Mouse); 308909 (Rat)

Alternate Names

Acid sphingomyelinase; aSMase; ASMsphingomyelin phosphodiesterase; EC 3.1.4.12; NPD; SMPD1; sphingomyelin phosphodiesterase 1, acid lysosomal

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human SMPD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Acid sphingomyelinase expression is associated with survival in resectable pancreatic ductal adenocarcinoma
Authors: Gregory C. Wilson, Sameer H. Patel, Jiang Wang, Kui Xu, Kevin M. Turner, Katrin Anne Becker et al.
Journal of Molecular Medicine
Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins
Authors: Xiahui Xiong, Chia-Fang Lee, Wenjing Li, Jiekai Yu, Linyu Zhu, Yongsoon Kim et al.
Biology Open
Ceramide-induced cleavage of GPR64 intracellular domain drives Ewing sarcoma
Authors: Suvarna, K;Jayabal, P;Ma, X;Wang, H;Chen, Y;Weintraub, ST;Han, X;Houghton, PJ;Shiio, Y;
Cell reports
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Enrichment of NPC1-deficient cells with the lipid LBPA stimulates autophagy, improves lysosomal function, and reduces cholesterol storage
Authors: O Ilnytska, K Lai, K Gorshkov, ML Schultz, BN Tran, M Jeziorek, TJ Kunkel, RD Azaria, HS McLoughlin, M Waghalter, Y Xu, M Schlame, N Altan-Bonn, W Zheng, AP Lieberman, R Dobrowolsk, J Storch
The Journal of Biological Chemistry, 2021-05-21;0(0):100813.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Acid sphingomyelinase deactivation post-ischemia promotes brain angiogenesis and remodeling by small extracellular vesicles
Authors: Ayan Mohamud Yusuf, Nina Hagemann, Xiaoni Zhang, Maria Zafar, Tanja Hussner, Carolin Bromkamp et al.
Basic Research in Cardiology