Human SNAP29 Antibody

Catalog #: MAB7869 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7869
MAB7869-SP

Detection of Human SNAP29 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SNAP29 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SNAP29 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 827003

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SNAP29
Met1-Glu129
Accession # O95721

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SNAP29 by Western Blot. Western blot shows lysates of SK-Mel-28 human malignant melanoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SNAP29 Monoclonal Antibody (Catalog # MAB7869) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SNAP29 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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SNAP29 in HepG2 Human Cell Line. SNAP29 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human SNAP29 Monoclonal Antibody (Catalog # MAB7869) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SNAP29

SNAP29 (29 kDa Soluble NSF [n-ethylmaleimide sensitive factor] Attachment Protein; also Synaptosomal associated protein 29 and GS32 [in rodent]) is a cytosolic and membrane-associated 27-32 kDa member of the syntaxin/ SNAP-25 family of proteins. It is widely, if not ubiquitously, expressed, and found in cell types as diverse as oligodentroglia, mast cells, neurons, Schwann cells and likely keratinocytes. Functionally, SNAP29 interacts with directly with syntaxin-1A and negatively impacts neurotransmission by inhibiting SNARE complex disassembly. In addition, it interacts with EHD1and AP-2, contributing to receptor-mediated endocytosis. Finally, it also might be said that SNAP29 is a key to the maintenance of general intracellular trafficking patterns. In this regard, SNAP29 has a remarkable ability to bind to a large number of syntaxins associated with multiple internal membranes. Human SNAP29 is 258 amino acids (aa) in length. It contains one t-SNARE coiled-coil homology domain (aa 196-258). Over aa 1-129, human and mouse SNAP29 share 88% aa sequence identity.

Long Name

Synaptosomal-associated Protein, 29 kDa

Entrez Gene IDs

9342 (Human); 67474 (Mouse); 116500 (Rat)

Alternate Names

CEDNIK; CEDNIKSoluble 29 kDa NSF attachment protein; SNAP29; SNAP-29FLJ21051; synaptosomal-associated protein 29; synaptosomal-associated protein, 29kD; synaptosomal-associated protein, 29kDa; Vesicle-membrane fusion protein SNAP-29