Human SOCS-7/Nck/NAP4 Antibody

Catalog #: MAB5809
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5809
MAB5809-SP

Detection of Human SOCS‑7/Nck/NAP4 by Western Blot.

1 Image

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOCS-7/Nck/NAP4 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human SOCS-7 in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 519106

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human SOCS-7
His351-Thr581
Accession # O14512

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human SOCS‑7/Nck/NAP4 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF-7 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human SOCS-7/Nck/NAP4 Monoclonal Antibody (Catalog # MAB5809) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SOCS-7/Nck/NAP4 at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-7/Nck/NAP4

Suppressor of cytokine signaling 7 (SOCS-7; also Nck, Ash, and phospholipase C gamma-binding protein and Nck-associated protein 4) is a 63 kDa member of the SOCS family of proteins. It regulates signaling cascades through protein ubiquitination and/or sequestration. Human SOCS-7 is 581 amino acids (aa) in length and contains four poly-Pro regions, a poly-Gly region, a poly-Gln region, an SH2 domain, and a SOCS box domain. A second isoform for SOCS-7 has a deletion of aa 1‑250 found in isoform 1. Human SOCS-7 is 97% aa identical to mouse SOCS-7. SOCS-7 is constitutively expressed in several tissues, most strongly in the testis and brain.

Long Name

Suppressor Of Cytokine Signaling 7

Entrez Gene IDs

30837 (Human); 192157 (Mouse); 287659 (Rat)

Alternate Names

NAP4; NAP-4; NAP4SOCS-7; Nck; Nck, Ash and phospholipase C binding protein; Nck, Ash and phospholipase C gamma-binding protein; NCKAP4; Nck-associated protein 4; SOCS6; SOCS7; SOCS-7; suppressor of cytokine signaling 7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human SOCS-7/Nck/NAP4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1