Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Soluble gp130 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours or 5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
0.08 ng/mL
Assay Range
0.1 - 8 ng/mL (Cell Culture Supernates), 0.3 - 16 ng/mL (Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sgp130
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human soluble gp130 Immunoassay is a 4.5 or 5.5 hour solid phase ELISA designed to measure sgp130 in cell culture supernates, serum, and plasma. It contains soluble insect cell-expressed recombinant human gp130 and antibodies raised against recombinant human gp130 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural gp130 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural gp130.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.4 | 1 | 4.43 | 0.4 | 1.02 | 4.5 |
| Standard Deviation | 0.02 | 0.07 | 0.33 | 0.02 | 0.1 | 0.31 |
| CV% | 5 | 7 | 7.4 | 5 | 9.8 | 6.9 |
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 0.7 | 1.82 | 8.43 | 0.77 | 1.94 | 8.64 |
| Standard Deviation | 0.03 | 0.1 | 0.4 | 0.04 | 0.07 | 0.3 |
| CV% | 4.3 | 5.5 | 4.7 | 5.2 | 3.6 | 3.5 |
Recovery
The recovery of sgp130 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 109 | 96-114 |
| Citrate Plasma (n=5) | 98 | 84-112 |
| EDTA Plasma (n=5) | 100 | 83-114 |
| Heparin Plasma (n=5) | 98 | 85-110 |
| Serum (n=5) | 101 | 83-120 |
Linearity
To assess the linearity of the assay, five samples were spiked with high concentrations of sgp130 in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the classI cytokine receptor family. Binding of IL-6 or IL-11 to either the membrane-anchored or soluble IL-6 R or IL-11 R, respectively, initiates the association of IL-6 R (IL-11 R) with gp130. The complex then undergoes homodimerization and signal transduction. For other IL-6 family cytokines, such as LIF and OSM, signal transduction is similarly triggered by the heterodimerization of gp130 with LIFR or OSM R.
Long Name:
Glycoprotein 130
Entrez Gene IDs:
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names:
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well.
- For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 1 hour.
For Cell Culture Supernate Samples: Cover with a new plate sealer, and incubate at room temperature for 2 hours. - Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
100 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Soluble gp130 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
14
Citations: Showing 1 - 10
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The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 signaling through shedding of the receptor gp130
Authors: SA Müller, MD Shmueli, X Feng, J Tüshaus, N Schumacher, R Clark, BE Smith, A Chi, S Rose-John, ME Kennedy, SF Lichtentha
Molecular Neurodegeneration, 2023-02-21;18(1):13.
Species: Human
Sample Types: CSF
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Circulating Microvesicles in Association with the NLRP3 Inflammasome in Coronary Thrombi from STEMI Patients
Authors: V Bratseth, J Nordeng, R Helseth, S Solheim, S Åkra, H Arnesen, G Chiva-Blan, I Seljeflot
Biomedicines, 2022-09-05;10(9):.
Species: Human
Sample Types: Plasma
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Co-Occurrence of Interleukin-6 Receptor Asp358Ala Variant and High Plasma Levels of IL-6: An Evidence of IL-6 Trans-Signaling Activation in Deep Vein Thrombosis (DVT) Patients
Authors: R Salemi, G Gattuso, B Tomasello, A Lavoro, A Gaudio, M Libra, SS Signorelli, S Candido
Biomolecules, 2022-05-10;12(5):.
Species: Human
Sample Types: Plasma
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The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI
Authors: J Nordeng, H Schandiz, S Solheim, S Åkra, P Hoffman, B Roald, B Bendz, H Arnesen, R Helseth, I Seljeflot
Mediators of Inflammation, 2021-05-27;2021(0):5525917.
Species: Human
Sample Types: Serum
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Inhibitory Mechanism of the Outer Membrane Growth of Chronic Subdural Hematomas
Authors: Koji Osuka
J. Neurotrauma, 2017-02-27;0(0):.
Species: Human
Sample Types: Chronic Subdural Hematoma Fluids
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Maternal country of origin, breast milk characteristics and potential influences on immunity in offspring.
Authors: Holmlund U, Amoudruz P, Johansson MA, Haileselassie Y, Ongoiba A, Kayentao K, Traore B, Doumbo S, Schollin J, Doumbo O, Montgomery SM, Sverremark-Ekstrom E
Clin. Exp. Immunol., 2010-10-05;162(3):500-9.
Species: Human
Sample Types: Milk
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Serum soluble glycoprotein 130 concentration is inversely related to insulin sensitivity in women with polycystic ovary syndrome.
Authors: Nikolajuk A, Kowalska I, Karczewska-Kupczewska M, Adamska A, Otziomek E, Wolczynski S, Kinalska I, Gorska M, Straczkowski M
Diabetes, 2010-01-26;59(4):1026-9.
Species: Human
Sample Types: Serum
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Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease.
Authors: Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A
Int. J. Cancer, 2009-01-15;124(2):367-75.
Species: Human
Sample Types: Plasma
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Functional characterization of a soluble gp130 isoform and its therapeutic capacity in an experimental model of inflammatory arthritis.
Authors: Richards PJ, Nowell MA, Horiuchi S, McLoughlin RM, Fielding CA, Grau S, Yamamoto N, Ehrmann M, Rose-John S, Williams AS, Topley N, Jones SA
Arthritis Rheum., 2006-05-01;54(5):1662-72.
Species: Human
Sample Types: Serum
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Circulating interleukin-6 family cytokines and their receptors in patients with congestive heart failure.
Authors: Hirota H, Izumi M, Hamaguchi T, Sugiyama S, Murakami E, Kunisada K, Fujio Y, Oshima Y, Nakaoka Y, Yamauchi-Takihara K
Heart Vessels, 2004-09-01;19(5):237-41.
Species: Human
Sample Types: Plasma
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Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Authors: Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
J. Virol., 2003-02-01;77(3):1727-37.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Serum
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Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells.
Authors: Erices A, Conget P, Rojas C, Minguell JJ
Exp. Cell Res., 2002-10-15;280(1):24-32.
Species: Human
Sample Types: Cell Culture Supernates
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Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease.
Authors: Hampel H, Teipel SJ, Padberg F, Haslinger A, Riemenschneider M, Schwarz MJ, Kotter HU, Scheloske M, Buch K, Stubner S, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ
Brain Res., 1999-03-27;823(1):104-12.
Species: Human
Sample Types: CSF
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Interleukin-6 and the soluble IL-6 receptor are decreased in cerebrospinal fluid of geriatric patients with major depression: no alteration of soluble gp130.
Authors: Stubner S, Schon T, Padberg F, Teipel SJ, Schwarz MJ, Haslinger A, Buch K, Dukoff R, Lasser R, Moller N, Sunderland T, Rapoport SI, Moller HJ, Hampel H
Neurosci. Lett., 1999-01-15;259(3):145-8.
Species: Human
Sample Types: CSF
FAQs
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Reviews for Human Soluble gp130 Quantikine ELISA Kit
Average Rating: 5 (Based on 4 Reviews)
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Human Soluble gp130 Quantikine ELISA Kit
By Anonymous
on 01/07/2022
Sample Tested: A375s2 human melanoma cell line, subclone 2
Human Soluble gp130 Quantikine ELISA Kit
By Anonymous
on 01/07/2020
Sample Tested: Cell culture supernatant,Serum and Plasma
Human Soluble gp130 Quantikine ELISA Kit
By Anonymous
on 11/07/2019
Sample Tested: Cell Culture Supernates
Human Soluble gp130 Quantikine ELISA Kit
By Jessica Fernando
on 12/04/2017
Sample Tested: Serum
