Human SorLA Antibody

Catalog #: MAB5699
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5699
MAB5699-SP

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All SorLA Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SorLA Antibody Summary

Species Reactivity

Human

Specificity

Detects human SorLA in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 525122

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SorLA
Ser82-Ser1550
Accession # Q92673

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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SorLA in Human Brain. SorLA was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human SorLA Monoclonal Antibody (Catalog # MAB5699) at 25 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasmic granules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry

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SorLA in Human Brain. SorLA was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human SorLA Monoclonal Antibody (Catalog # MAB5699) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SorLA

SorLA (sortilin-related receptor, LDLR class A repeats-containing), also called LR11 or SORL1, is a 250 kDa type I transmembrane glycoprotein of the Sortilin family of Vps10p-domain receptors. SorLA is found in both intracellular and surface membranes in the central nervous system, and mediates trafficking of proteins such as the amyloid precursor protein (APP). The 2214 amino acid (aa) human SorLA includes a 28 aa signal sequence, a 53 aa furin-cleaved propeptide, and a 2056 aa extracellular domain. The region used as an immunogen, which includes BNR, LDLR class A and B, and EGF-like domains, but excludes fibronectin-like domains, shares 95% aa identity with mouse and rat SorLA.

Long Name

Sortilin-related Receptor Containing LDLR Class A Repeats

Entrez Gene IDs

6653 (Human); 20660 (Mouse); 300652 (Rat)

Alternate Names

C11orf32; chromosome 11 open reading frame 32; FLJ21930; FLJ39258; gp250; Low-density lipoprotein receptor relative with 11 ligand-binding repeats; LR11; LR11LDLR relative with 11 ligand-binding repeats; LRP9; mosaic protein LR11; SORL1; SorLA; SorLA-1SORLA; sortilin-related receptor; sortilin-related receptor, L(DLR class) A repeats containing; sortilin-related receptor, L(DLR class) A repeats-containing; Sorting protein-related receptor containing LDLR class A repeats

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human SorLA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Expression of an alternatively spliced variant of SORL1 in neuronal dendrites is decreased in patients with Alzheimer's disease
Authors: G Monti, M Kjolby, AMG Jensen, M Allen, J Reiche, PL Møller, R Comaposada, BE Zolkowski, C Vieira, MM Jørgensen, IE Holm, PN Valdmanis, N Wellner, CB Vægter, SJ Lincoln, A Nykjær, N Ertekin-Ta, JE Young, M Nyegaard, OM Andersen
Acta neuropathologica communications, 2021-03-16;9(1):43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Serum low-density lipoprotein and low-density lipoprotein expression level at diagnosis are favorable prognostic factors in patients with small-cell lung cancer (SCLC)
Authors: T Zhou, J Zhan, W Fang, Y Zhao, Y Yang, X Hou, Z Zhang, X He, Y Zhang, Y Huang, L Zhang
BMC Cancer, 2017-04-14;17(1):269.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P