Human SOX13 Antibody

Catalog #: MAB5118 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB5118-100
MAB5118-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SOX13 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SOX13 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2559A

Purification

Protein A or G purified from cell culture supernatant

Immunogen

E. coli-derived recombinant human SOX13
Met19-Pro258
Accession # Q9UN79

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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SOX13 in Human Pancreas. SOX13 was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human SOX13 Monoclonal Antibody (Catalog # MAB5118) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei and cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX13

SOX-13 is a transcription factor involved in the embryonic development and the determination of cell fate. It is also known as islet cell antibody 12 since it has been determined to be a type-1 diabetes autoantigen. SOX-13 RNA is detected in most human tissues with highest levels in the pancreas, placenta, and kidney. Immunohistochemistry on human pancreas identifies SOX-13 in the islets of Langerhans where staining is mostly cytoplasmic but can also be nuclear. SOX-13 antibody testing helps identify latent autoimmune diabetes of adults (LADA).

Long Name

SRY (sex determining region Y)-box 13

Entrez Gene IDs

9580 (Human); 20668 (Mouse); 289026 (Rat)

Alternate Names

ICA12; Islet cell antigen 12; MGC117216; SOX13; Sox-13; SRY (sex determining region Y)-box 13ICA12islet cell 12; SRY-box 13; SRY-related HMG-box gene 13; transcription factor SOX-13; Type 1 diabetes autoantigen ICA12