Human SOX6 Antibody Summary
Met1-Leu339
Accession # P35712
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of SOX6 in Human Liver Cancer. SOX6 was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Mouse Anti-Human SOX6 Monoclonal Antibody (Catalog # MAB77591) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX6
SOX6 is a 92 kDa member of the Sox [Sry-related high mobility group (HMG) box] DNA binding protein family, and initially was isolated from an adult testis cDNA library. Human SOX6 is 828 amino acids (aa) in length. Aa 184-262 constitute a coiled-coil region. Aa 219-261, 280-285, and 313-317 make up a Glu-rich and two poly-Ala regions, respectively. Also, there are two additional isoforms for SOX6. Isoform 2 is formed by the deletion of aa 327-367 found in isoform 1, and isoform 3 is formed by the deletion of aa 579-598 found in isoform 1. Finally, aa 620-683 make up the SOX-TCF-HMG-box region. Human SOX6 shares 97% aa identity with mouse SOX6. Previous studies have suggested that SOX6 plays a role in the development of the central nervous system (CNS) and chondrogenesis. Another study, however, revealed that the mutant pIOOH allele, which is located on the same chromosome as SOX6, develops myopathy and an atrioventricular (AV) heart block, a cardiac conduction defect that is a main cause of death in human cardiac myopathies. Electronmicroscopic evaluation of the mutant cardiac and skeletal muscle demonstrated significant change in ultrastructure. Thus, the phenotype of the pIOOH mutation suggests that the SOX6 protein also may be involved in maintaining normal physiological functions of muscle tissue, including the heart. In addition genome-wide association studies have found that the SOX6 gene plays an important role in the coregulation of obesity and osteoporosis. Moreover, SOX6 has been shown to be a transcriptional factor that is specifically expressed in the developing nervous system and in the early stages of chondrogenesis in mouse embryos, and it has been revealed that SOX6 was expressed in glioma tissues, but not in normal adult brain tissue.
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