Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SPRED1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SPRED1
Arg118-Lys324
Accession # Q7Z699

Specificity

Detects endogenous human SPRED1 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human SPRED1 Antibody

Detection of Human SPRED1 antibody by Western Blot.

Detection of Human SPRED1 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and human brain tissue. PVDF membrane was probed with 1 µg/mL of Human SPRED1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5067) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED1 at approximately 55 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human SPRED1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and human brain tissue

Reviewed Applications

Read 2 reviews rated 3.5 using AF5067 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SPRED1

SPRED1 (Sprouty-related protein with an EVH1 domain-1) is a 55 kDa member of the SPRED family of regulatory molecules. It inhibits mitogenic signaling by blocking Ras activation of Raf, and disrupts actin stress fiber formation by binding TESK1. Human SPRED1 is 444 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 6‑123), a central KBD domain that binds to the SCFR (aa 233‑285), and a C-terminal Cys‑rich/Sprouty-related domain that mediates homo- and heterodimerization with SPRED2, binding to TESK1, and undergoes palmitoylation for membrane localization (aa 334‑442). There is one splice variant that shows a nine Lys substitution for aa 269‑444.

Long Name

Sprouty-related with an EVH1 Domain Containing-1

Alternate Names

NFLS

Entrez Gene IDs

161742 (Human); 114715 (Mouse); 296072 (Rat)

Gene Symbol

SPRED1

UniProt

Q7Z699

Additional SPRED1 Products

Product Documents for Human SPRED1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human SPRED1 Antibody

For research use only

Citations for Human SPRED1 Antibody

Customer Reviews for Human SPRED1 Antibody (2)

3.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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3 Stars
50%
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1 Stars
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  • Human SPRED1 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human SPRED1 Antibody AF5067
  • Human SPRED1 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/02/2018

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