Human SPRY4 Antibody

Detection of Human SPRY4 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRY4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human SPRY4 by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SPRY4 at approximately 45 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human SPRY4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5070) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Human SPRY4 by Western Blot KSRP is a novel regulator of Spry4.A and B, total RNA from H2122 and H157 cells treated with KSRP-specific siRNAs was isolated, and the expression of Spry4 was later determined by qPCR as described under “Experimental procedures.” **, p < 0.01, t test. C, total RNA from Beas2B cells transiently transfected with either control or FLAG-KSRP expression vectors was isolated, and the expression of Spry4 was later determined by qPCR as described under “Experimental procedures.” *, p < 0.05, t test. D, total RNA from H2122 clones with stable expression of control shRNA or KSRP shRNA was isolated, and the expression of Spry4 was later determined by qPCR as described under “Experimental procedures.” *, p < 0.05; **, p < 0.01, ANOVA. E and F, proteins from H2122 and H157 cells treated with KSRP-specific siRNAs were isolated, and the expression of Spry4 was later determined by immunoblotting. Representative data of two independent, highly reproducible experiments are displayed. Ctrl, control; IB, immunoblotting. Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S0021925820428558), licensed under a CC-BY license. Not internally tested by R&D Systems.