Human SRCRB4D Antibody

Catalog #: AF5654 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5654
AF5654-SP

Detection of Human SRCRB4D by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SRCRB4D Antibody Summary

Species Reactivity

Human

Specificity

Detects human SRCRB4D in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human CD163 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human SRCRB4D isoform 1
Ala45-Ser575
Accession # Q8WTU2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SRCRB4D by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SRCRB4D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5654) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SRCRB4D at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SRCRB4D

AMSH (Associated molecule with SH3 domain of STAM) is a 50 kDa member of the peptidase M67C class of enzymes. It is widely expressed and serves two functions. First, it promotes receptor recycling by counteracting the effects of ubiquitinating enzymes, and second, it participates in cytokine signal transduction by forming a complex with Jak2/3, STAM and the common beta ‑ and gamma ‑chains. Human AMSH is 424 amino acids (aa) in length. It contains an NLS (aa 113‑127), two SH3‑binding motifs (aa 195‑198 and 227‑231), and one MPN domain (aa 252‑361). There are five phosphorylation sites at Ser2, 48, 243, 245 and 247. AMSH has one potential splice variant that shows a 17 aa substitution for aa 1‑68. Full‑length human AMSH is 83% aa identical to mouse AMSH.

Long Name

Scavenger Receptor Cysteine-rich Domain Containing Group B

Entrez Gene IDs

136853 (Human); 109267 (Mouse); 304401 (Rat)

Alternate Names

S4D-SRCRB; S4-SRCRB; scavenger receptor cysteine rich domain containing, group B (4 domains); scavenger receptor cysteine-rich domain-containing group B protein; scavenger receptor cysteine-rich protein SRCRB-S4D; SRCRB4D; SRCRB-S4D; SSC4D