Human SRPK3 Antibody

Catalog #: MAB7230
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7230
MAB7230-SP

Detection of Human SRPK3 by Western Blot.

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All SRPK3 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human SRPK3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SRPK3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) SRPK1 or rhSRPK2 is observed.

Source

Monoclonal Mouse IgG_2B Clone # 722534

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E.coli-derived recombinant human SRPK3
Arg247-Ser316
Accession # Q9UPE1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SRPK3 by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SRPK3 Monoclonal Antibody (Catalog # MAB7230) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for SRPK3 at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SRPK3

SRPK3 (Ser/Arg-rich protein specific kinase 3), also called MSSK-1 (muscle-specific serine kinase 1) or STK23 (Ser/Thr kinase 23) is a 59 kDa (predicted) cytoplasmic member of the CMGC Ser/Thr protein kinase family. SRPK3 is principally expressed in heart and skeletal muscle. SRPKs selectively phosphorylate Ser on RS domain-containing proteins, initiating mRNA splicing and maturation. The 533 amino acid (aa) human SRPK3 contains a split kinase domain (aa 79‑292 and 347‑531) separated by a hinge region. A 491 aa isoform shows an alternate translation start site at aa 43. Within the region used as an immunogen, human SRPK3 shares 87% and 86% aa identity with mouse and rat SRPK3, respectively.

Long Name

Ser/Arg-rich Protein-specific Kinase 3

Entrez Gene IDs

26576 (Human); 56504 (Mouse); 293854 (Rat)

Alternate Names

MGC102944; MSSK1; MSSK-1; MSSK1EC 2.7.11.1; Muscle-specific serine kinase 1; serine arginine rich protein-specific kinase 3; Serine/arginine-rich protein-specific kinase 3; serine/threonine kinase 23; Serine/threonine-protein kinase 23; serine/threonine-protein kinase SRPK3; SFRS protein kinase 3; SRPK3; SR-protein-specific kinase 3; SRSF protein kinase 3; STK23

Product Datasheets

Product Datasheet

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Citation for Human SRPK3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1