Human STAT2 Antibody

Catalog #: MAB16662 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB16662-100
MAB16662-SP

Detection of Human STAT2 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human STAT2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human STAT2 in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 967425

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human STAT2
Gln679-Phe851
Accession # P52630

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Knockout Validated

STAT2 is specifically detected in HeLa human cervical epithelialcarcinoma parental cell line but is not detectable in STAT2knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human STAT2 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16662) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human STAT2 by Simple Western^TM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for STAT2 at approximately 110 kDa (as indicated) using 10 µg/mL of Rat Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16662) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated

Western Blot Show Human STAT2 Antibody Specificity Using Knockout Cell Line.

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Western Blot Show Human STAT2 Specificity Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and STAT2 knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Rat Anti-Human STAT2 Monoclonal Antibody (Catalog # MAB16662) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT2 at approximately 110 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT2

Signal transducer and activator of transcription 2 (STAT2) is a member of the transcription factor STAT family with an apparent M.W. of 113 kDa in SDS-PAGE gels. Human STAT2 is 851 amino acids (aa) in length and contains one SH2 domain (aa 572-667). Splicing variants produce two isoforms for human STAT2. The short isoform has a 32 aa substitution and a 199 aa deletion corresponding to aa 621‑652 and aa 653‑851 in the long isoform, respectively. Human STAT2 shares 73% and 65% aa sequence identity with rat and mouse STAT2, in that order. STAT2 functions as a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta ), and it is also required for myogenic differentiation.

Long Name

Signal Transducer and Activator of Transcription 2

Entrez Gene IDs

6773 (Human); 20847 (Mouse)

Alternate Names

interferon alpha induced transcriptional activator; ISGF-3; MGC59816; P113; signal transducer and activator of transcription 2; signal transducer and activator of transcription 2, 113kD; signal transducer and activator of transcription 2, 113kDa; STAT113; STAT2