Human STEAP1 APC‑conjugated Antibody

R&D Systems | Catalog # FAB55871A

R&D Systems
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rabbit IgG Clone # 2959F
View all STEAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human STEAP1
Met1-Trp71
Accession # Q9UHE8

Specificity

Detects human STEAP1 in direct ELISA.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human STEAP1 APC‑conjugated Antibody

Detection of STEAP1 in HEK293 cells by Flow Cytometry.

HEK293 cells transfected with Human STEAP-1 and eGFP (A) vs irrelevant cells (B) were stained with Rabbit Anti-Human STEAP1 APC-conjugated MonoclonalAntibody (Catalog # FAB55871A). View our protocol for Staining Membrane-associated Proteins.

Detection of STEAP1 in LnCaP cells by Flow Cytometry

LnCaP cells were stained with Rabbit Anti-Human STEAP1 APC‑conjugated Monoclonal Antibody (Catalog # FAB55871A, filled histogram) or isotype control antibody (Catalog # IC1051A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with saponin. View our protocol for Staining Intracellular Molecules.

Applications for Human STEAP1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: see below

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STEAP1

STEAP1 (six-transmembrane epithelial antigen of the prostate-1) is a 40 kDa protein (predicted) of the STEAP family of metalloreductases. It is expressed mainly at cell‑cell junctions between prostate secretory epithelium, and up‑regulated in prostate and some bladder, colon and ovarian cancers and Ewing’s sarcomas. Human STEAP1 is 339 amino acids (aa) in length. It contains a ferric oxidoreductase domain (aa 119‑265) that includes transmembrane sequences (# 2, 3, 4, and part of 5, out of 6 sequences). Two splice forms diverge at aa 255, terminating after aa 58 or 259. Over aa 1‑71, human and mouse STEAP1 share 68% aa identity.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Alternate Names

PRSS24

Entrez Gene IDs

26872 (Human); 70358 (Mouse); 297738 (Rat)

Gene Symbol

STEAP1

UniProt

Q9UHE8 (Human)

Additional STEAP1 Products

Product Documents for Human STEAP1 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human STEAP1 APC‑conjugated Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Redox Enzymes

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