Human SVEP1 Antibody

Catalog #: MAB9774 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9774-100
MAB9774-SP

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Human SVEP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SVEP1 in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 982903

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human SVEP1 peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

5-25 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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SVEP1 in MG‑63 Human Cell Line. SVEP1 was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB9774) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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SVEP1 in Human Placenta. SVEP1 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB9774) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblasts. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SVEP1

Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (also known as Polydom), is a 3571 aminoacids (aa), 390KDa extracellular matrix protein encoded by the SVEP1 gene. SVEP1 has been identified as a multidomain cell adhesion protein that is a ligand for integrin alpha 9 beta 1. SVEP1 has been proposed to play a crucial role in epidermis differentiation and lymphatic remodeling.

Long Name

Sushi, Von Willebrand Factor Type A, EGF And Pentraxin Domain Containing 1

Entrez Gene IDs

79987 (Human); 64817 (Mouse); 685899 (Rat)

Alternate Names

C9orf13; CCP module-containing protein 22; CCP22; FLJ16013; FLJ90719; POLYDOM; selectin-like protein; SELOB; SEL-OB; sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1; sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1; SVEP1