Human SVEP1 Antibody

Catalog #: MAB97741 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB97741-100
MAB97741-SP

Detection of Human SVEP1 by Western Blot.

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Human SVEP1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human SVEP1 in direct Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 987420

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide containing human SVEP1 Peptide
Accession # Q4LDE5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human SVEP1 by Western Blot. Western blot shows lysates of MG-63 human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SVEP1 Monoclonal Antibody (Catalog # MAB97741) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SVEP1 at approximately 460 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SVEP1

Sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1 (also known as Polydom), is a 3571 aminoacids (aa), 390KDa extracellular matrix protein encoded by the SVEP1 gene. SVEP1 has been identified as a multidomain cell adhesion protein that is a ligand for integrin alpha 9 beta 1. SVEP1 has been proposed to play a crucial role in epidermis differentiation and lymphatic remodeling.

Long Name

Sushi, Von Willebrand Factor Type A, EGF And Pentraxin Domain Containing 1

Entrez Gene IDs

79987 (Human); 64817 (Mouse); 685899 (Rat)

Alternate Names

C9orf13; CCP module-containing protein 22; CCP22; FLJ16013; FLJ90719; POLYDOM; selectin-like protein; SELOB; SEL-OB; sushi, von Willebrand factor type A, EGF and pentraxin domain containing 1; sushi, von Willebrand factor type A, EGF and pentraxin domain-containing protein 1; SVEP1