Human Syndecan-4 Antibody

Catalog #: MAB29181 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29181-100
MAB29181-SP

Detection of Syndecan-4 in Human HeLa Cell Line by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Syndecan-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Syndecan-4 in direct ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 336304

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Syndecan-4
Ser20-Glu145
Accession # P31431

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Syndecan-4 in Human HeLa Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Syndecan-4 Monoclonal Antibody (Catalog # MAB29181, filled histogram) or Rat IgG2A isotype control antibody (Catalog # MAB006, open histogram) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-4

Syndecan-4, previously known as Amphiglycan or Ryudocan, is a member of the syndecan family of Type 1 transmembrane proteins capable of carrying Heparan Sulfate (HS) and Chondroitin Sulfate (CS) glycosaminoglycans. The four vertebrate syndecans have two conserved cytoplasmic domains and divergent extracellular portions, except for HS attachment sites. Syndecan-4 is the most similar to Syndecan-2, but is more universally expressed and is found in virtually every cell type. Expression can be upregulated by TGF-beta 2 and in response to mechanical stress in smooth muscle, wound healing, arterial injury or acute myocardial infarction, probably in response to at least one inflammatory mediator (1, 2). Human Syndecan-4 is synthesized as a 198 amino acid (aa) core protein with an 18 aa signal sequence, a 127 aa extracellular domain containing three consensus Ser-Gly sequences for the attachment of HS side chains, a 25 aa transmembrane region and a 28 aa cytoplasmic tail (3). Human Syndecan-4 ECD shares approximately 79%, 78% and 81% aa sequence identity with mouse, rat and porcine Syndecan-4 ECD, respectively. Addition of 20‑80 disaccharides per side chain adds considerably to the size of the 20 kDa core protein. Non-covalent homodimerization of Syndecan-4 is dependent on the transmembrane domain (4). The HS chains can bind fibronectin, SDF-1, antithrombin, FGF-2, midkine and tissue factor pathway inhibitor and can present FGF‑2 to its receptors (1, 2, 5). Proteolytic cleavage by plasmin, thrombin or a metalloproteinase may create a functional ectodomain (6‑8). Genetic disruption of the Syndecan-4 gene causes a mild phenotype, presumably due to compensation by other syndecans, but mice have an increase in placental thrombi as well as defects in wound healing and response to endotoxin shock (9, 10).

References

Tkachenko, E. et al. (2005) Circ. Res. 96:488.
Oh, E.-S, and J. R. Couchman (2004) Mol. Cells 17:181.
David, G. et al. (1992) J. Cell Biol. 118:961.
Choi, S. et al. (2005) J. Biol. Chem. 280:42573.
Charnaux, N. et al. (2005) FEBS J. 272:1937.
Schmidt, A. et al., J. Biol. Chem. 280:34441.
Rauch, B. H. et al. (2005) J. Biol. Chem. 280:17507.
Fitzgerald, M. L. et al. (2000) J. Cell Biol. 148:811.
Ishiguro, K. et al. (2003) Glycoconj. J. 19:315.
Echtermeyer, F. et al. (2001) J. Clin. Invest. 107:R9.

Entrez Gene IDs

6385 (Human); 20971 (Mouse)

Alternate Names

Amphiglycan; MGC22217; Ryudocan core protein; Ryudocan; SDC4; SYND4ryudocan amphiglycan; syndecan 4 (amphiglycan, ryudocan); syndecan 4; syndecan proteoglycan 4; Syndecan4; Syndecan-4