Human TACI/TNFRSF13B Antibody

Name: Human TACI/TNFRSF13B Antibody
Brand: R&D Systems
SKU: MAB1741
Rating: 5 (1 reviews)

Catalog #: MAB1741
3 Citations 1 Review Datasheet / COA / SDS

Clone 165604 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
MAB1741
MAB1741-SP

Detection of TACI/TNFRSF13B in Raji Human Cell Line by Flow Cytometry.

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Product Details

Citations (3)

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Reviews (1)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TACI/TNFRSF13B Antibody Summary

Species Reactivity

Human

Specificity

Detects human TACI/TNFRSF13B in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human BCMA or recombinant mouse TACI is observed.

Source

Monoclonal Mouse IgG₁ Clone # 165604

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # O14836

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.05-0.2 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TACI/TNFRSF13B in Raji Human Cell Line by Flow Cytometry. Raji human Burkitt's lymphoma cell line was stained with Mouse Anti-Human TACI/TNFRSF13B Monoclonal Antibody (Catalog # MAB1741, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PerCP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0114).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science, 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TACI/TNFRSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Macrophages express membrane bound form of APRIL that can generate immunomodulatory signals.
Authors: Lee SM, Jeon ST, Suk K, Lee WH
Immunology, 2010-11-01;131(3):350-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.
Authors: He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Doffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A
Nat. Immunol., 2010-08-01;11(9):836-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Neutralizing B-cell activating factor antibody improves survival and inhibits osteoclastogenesis in a severe combined immunodeficient human multiple myeloma model.
Authors: Neri P, Kumar S, Fulciniti MT, Vallet S, Chhetri S, Mukherjee S, Tai Y, Chauhan D, Tassone P, Venuta S, Munshi NC, Hideshima T, Anderson KC, Raje N
Clin. Cancer Res., 2007-10-01;13(19):5903-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry