Human TACI/TNFRSF13B Antibody

Catalog #: MAB174
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB174-SP
MAB174-500
MAB174-100

Detection of Human TACI/TNFRSF13B/CVID by Immunocytochemistry/Immunofluorescence

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Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TACI/TNFRSF13B Antibody Summary

Species Reactivity

Human

Specificity

Detects human TACI/TNFRSF13B in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse TACI or recombinant human BCMA is observed.

Source

Monoclonal Mouse IgG₁ Clone # 165609

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # O14836

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human spleen

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.03-0.12 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Human TACI/TNFRSF13B Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human TACI/TNFRSF13B Biotinylated Antibody (Catalog # BAF174)

Standard: Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF (Catalog # 174-TC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence

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Detection of Human TACI/TNFRSF13B/CVID by Immunocytochemistry/Immunofluorescence Expressions of BLyS, TACI, BCMA and BAFF-R in human breast cancer cell lines. (A) BLyS and its three receptors in human breast cancer cell lines MDA-MB-435, MDA-MB-231, MDA-MB-468 and B cell line Ramos by immunofluorescence (original magnification 200 ×) and Western Blotting. (B) The mRNA level of BLyS in the three cell lines were detected by real-time PCR under hypoxia for different time points. Data were means of triplicate samples with ± SD; vs normoxia, *, P < 0.05; **, P < 0.01; ***, P < 0.001. (C) BLyS protein level in MDA-MB-435 cells by Western Blotting analysis. Image collected and cropped by CiteAb from the following publication (https://jeccr.biomedcentral.com/articles/10.1186/1756-9966-31-31), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science, 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TACI/TNFRSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness
Authors: M Warakomska, M Tynecka, D Lemancewic, K Grubczak, J Dzieciol, M Moniuszko, A Eljaszewic, L Bolkun
Oncology Letters, 2021-08-10;22(4):728.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity.
Authors: Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E
J Immunol, 2014-12-10;194(2):542-52.
Species: Human
Sample Types: Cell Lysates, Protein, Whole Cells
Applications: Flow Cytometry, Immunoprecipitation, Western Blot
BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
Authors: Zhu J, Sun L, Lin S
J. Exp. Clin. Cancer Res., 2012-03-31;31(0):31.
Species: Human
Sample Types: Whole Cells
Applications: ICC