Human TACI/TNFRSF13B Antibody

Catalog #: AF174
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF174
AF174-SP

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Citations (6)

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Human TACI/TNFRSF13B Antibody Summary

Species Reactivity

Human

Specificity

Detects human TACI in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse TACI and recombinant human BCMA is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # Q53F36

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.075-0.3 µg/mL of this antibody will block 50% of the binding of 50 ng/mL of Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) to immobilized Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TACI/TNFRSF13B in Human Spleen. TACI/TNFRSF13B was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human TACI/TNFRSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF174) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to lymphocytes in the marginal zone. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular
N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

Xia, X-Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

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Product Datasheet

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Related Research Areas

Citations for Human TACI/TNFRSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways
Authors: M Imbrechts, K De Samblan, K Fierens, E Brisse, J Vandenhaut, T Mitera, C Libert, I Smets, A Goris, C Wouters, P Matthys
Eur. J. Immunol., 2018-08-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
Leukemia, 2015-07-03;30(1):163-72.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
The immunoregulator soluble TACI is released by ADAM10 and reflects B cell activation in autoimmunity.
Authors: Hoffmann F, Kuhn P, Laurent S, Hauck S, Berer K, Wendlinger S, Krumbholz M, Khademi M, Olsson T, Dreyling M, Pfister H, Alexander T, Hiepe F, Kumpfel T, Crawford H, Wekerle H, Hohlfeld R, Lichtenthaler S, Meinl E
J Immunol, 2014-12-10;194(2):542-52.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation
TACI mutations and impaired B-cell function in subjects with CVID and healthy heterozygotes.
Authors: Martinez-Gallo M, Radigan L, Almejun M, Martinez-Pomar N, Matamoros N, Cunningham-Rundles C
J Allergy Clin Immunol, 2012-12-11;131(2):468-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.
Authors: He B, Santamaria R, Xu W, Cols M, Chen K, Puga I, Shan M, Xiong H, Bussel JB, Chiu A, Puel A, Reichenbach J, Marodi L, Doffinger R, Vasconcelos J, Issekutz A, Krause J, Davies G, Li X, Grimbacher B, Plebani A, Meffre E, Picard C, Cunningham-Rundles C, Casanova JL, Cerutti A
Nat. Immunol., 2010-08-01;11(9):836-45.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot
Identification of transitional type II B cells in the salivary glands of patients with Sjogren's syndrome.
Authors: Daridon C, Pers JO, Devauchelle V, Martins-Carvalho C, Hutin P, Pennec YL, Saraux A, Youinou P
Arthritis Rheum., 2006-07-01;54(7):2280-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC