Human TACI/TNFRSF13B Biotinylated Antibody

Catalog #: BAF174
5 Citations Datasheet / COA / SDS

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BAF174

Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TACI/TNFRSF13B Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TACI/TNFRSF13B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant mouse TACI is observed and less than 0.2% cross-reactivity with recombinant human BCMA is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TACI/TNFRSF13B
Ser2-Thr166
Accession # Q53F36

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TACI/TNFRSF13B Fc Chimera (Catalog # 174-TC)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human spleen subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Human TACI/TNFRSF13B Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human TACI/TNFRSF13B Antibody (Catalog # MAB174)

Standard: Recombinant Human TACI/TNFRSF13B Fc Chimera Protein, CF (Catalog # 174-TC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TACI/TNFRSF13B

TACI, transmembrane activator and CAML-interactor, is a member of the TNF receptor superfamily. TACI is a type III membrane protein with an extracellular N‑terminus in the absence of a cleaved signal sequence. The extracellular region of TACI contains two cysteine rich domains. Within the TNFRSF, it shares the highest homology with BCMA. TACI and BCMA have both been shown to bind APRIL and BAFF, members of the TNF ligand superfamily. TACI is expressed on the cell surface of B cells and activated, but not resting, T cells. Analogous to BCMA, data suggests that TACI may play an important role in B cell development, function and regulation. Human TACI is a 293 amino acid (aa) protein consisting of a 166 aa extracellular domain, a 20 aa transmembrane domain, and a 107 aa intracellular domain. Human and mouse TACI share 54% amino acid identity.

References

Xia, X.-Z. et al. (2000) J. Exp. Med. 192:137.
von Bulow, G.U. et al. (1997) Science, 278:138.
Gross, J.A. et al. (2000) Nature 404:995.
Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
Yan, M. et al. (2000) Nature Immunol. 1:37.
Wu, Y. et al. (2000) J. Biol. Chem. 275:35478.

Long Name

Transmembrane Activator and CAML Interactor/Tumor Necrosis Factor Receptor Superfamily Member 13B

Entrez Gene IDs

23495 (Human); 57916 (Mouse); 102126451 (Cynomolgus Monkey)

Alternate Names

CD267 antigen; CD267; CVID; CVID2; FLJ39942; MGC133214; TACI; TACIMGC39952; TNFRSF13B; TNFRSF14B; Transmembrane activator and CAML interactor; tumor necrosis factor receptor 13B; tumor necrosis factor receptor superfamily member 13B; tumor necrosis factor receptor superfamily, member 13B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TACI/TNFRSF13B Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Fusion Toxin BLyS-Gelonin Inhibits Growth of Malignant Human B Cell Lines In Vitro and In Vivo.
Authors: Luster, Troy A, Mukherjee, Ipsita, Carrell, Jeffrey, Cho, Yun Hee, Gill, Jeffrey, Kelly, Lizbeth, Garcia, Andy, Ward, Christop, Oh, Luke, Ullrich, Stephen, Migone, Thi-Sau, Humphreys, Robin
PLoS ONE, 2012-10-09;7(10):e47361.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Atacicept in patients with rheumatoid arthritis: results of a multicenter, phase Ib, double-blind, placebo-controlled, dose-escalating, single- and repeated-dose study.
Authors: Tak PP, Thurlings RM, Rossier C, Nestorov I, Dimic A, Mircetic V, Rischmueller M, Nasonov E, Shmidt E, Emery P, Munafo A
Arthritis Rheum., 2008-01-01;58(1):61-72.
Species: Human
Sample Types: Serum
Applications: ELISA Development
IL-21 and BAFF/BLyS synergize in stimulating plasma cell differentiation from a unique population of human splenic memory B cells.
Authors: Ettinger R, Sims GP, Robbins R, Withers D, Fischer RT, Grammer AC, Kuchen S, Lipsky PE
J. Immunol., 2007-03-01;178(5):2872-82.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
Authors: Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM
Blood, 2005-11-22;107(7):2882-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Decreased survival of B cells of HIV-viremic patients mediated by altered expression of receptors of the TNF superfamily.
Authors: Moir S, Malaspina A, Pickeral OK, Donoghue ET, Vasquez J, Miller NJ, Krishnan SR, Planta MA, Turney JF, Justement JS, Kottilil S, Dybul M, Mican JM, Kovacs C, Chun TW, Birse CE, Fauci AS
J. Exp. Med., 2004-10-04;200(7):587-99.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry