Human TAPBPR Antibody

Catalog #: MAB11337 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11337-100
MAB11337-SP

Detection of TAPBPR in HEK293 cell transfected with Human TAPBL and eGFP vs irrelevant cells by Flow Cytometry.

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Human TAPBPR Antibody Summary

Species Reactivity

Human

Specificity

Dectects human TAPBPR in direct ELISA.

Source

Monoclonal Mouse IgG_2A Clone # 1059329

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese Hamster Ovary cell line, CHO-derived human TAPBPR
Ala19-Arg404
Accession # Q9BX59.2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

HEK293 cells transfected with Human TAPBL and eGFP vs irrelevant

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TAPBPR in HEK293 cell transfected with Human TAPBL and eGFP vs irrelevant cells by Flow Cytometry. HEK293 cells transfected with Human TAPBL and eGFP vs irrelevant were stained with eGFP and Mouse Anti-Human TAPBPR Monoclonal Antibody (A, B) (Catalog # MAB11337) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TAPBPR

TAP-binding protein-like (TAPBPL), also known as TAP binding protein-related (TAPBPR) and Tapasin-related protein (TAPASINR) is a transmembrane protein of the Immunoglobulin (Ig) superfamily (1, 2). TAPBPR was originally isolated as a homologue to TAPASIN but more recently was identified as a novel B7 family-related molecule since it shares sequence, structural, and functional similarities to B7 family members (3). Mature human TAPBPR consists of a lumenal domain containing an IgV and IgC domain, a transmembrane domain, and a cytoplasmic tail which lacks an ER retention motif. Within the lumenal domain, mature human TAPBPR shares 70% and 71% amino acid sequence identity with mouse and rat TAPBPR, respectively. Multiple alternatively spliced TAPBPR isoforms are known to exist with unique properties (4).TAPBPR is widely expressed and, similar to TAPASIN, functions as a both a chaperone protein and peptide editor of MHC class I, but in a peptide‑loading complex (PLC) independent manner (5, 6). TAPBPR decreases the rate at which MHC class I molecules mature through the secretory pathway, a role which could be important for peptide selection by MHC class I molecules (7). TAPBPR is also expressed on the surface of T cells and antigen-presenting cells (APCs) and plays an inhibitory role in the proliferation and activation of T cells (4). TAPBPR can be expressed on various cancer cells like leukemia and has the potential to be used in the treatment of autoimmune diseases and transplant rejection, as well as cancer (4).

References

Hermann, C. et al. (2015) Tissue antigens 85(3):155.
Teng, M. et al. (2002) European Journal of Immunology 32:1059.
Lin, Y. et al. (2021). EMBO Mol Med. 13(5):13404.
Porter, K.M. et al. (2014) Immunology 142:289.
Margulies, D. et al. (2020) Current Opinion in Immunology 64:71.
Boyle, L.H. et al. (2013) PNAS 110:3465.
Hermann, C. et al. (2013) Journal of Immunology 191(11):5743.

Long Name

Tapasin Related Protein

Entrez Gene IDs

55080 (Human); 213233 (Mouse); 297602 (Rat)

Alternate Names

FLJ10143; TAP binding protein-like; Tapasin Related Protein/TAPBPR; Tapasin-like; TAPASIN-R; tapasin-related protein; TAP-binding protein-like; TAP-binding protein-related protein; TAPBPL; TAPBPR; TAPBP-RTAP binding protein related; TAPBPRtapasin-like