Human TBK1 Antibody

Catalog #: AF9934 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF9934-100
AF9934-SP

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Human TBK1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TBK-1 in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TBK-1
Met1-Gly121
Accession # Q9UHD2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Simple Western

20 µg/mL

Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells

Immunohistochemistry

3-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TBK1 in Human Prostate Cancer Tissue. TBK1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western

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Detection of Human TBK1 by Simple Western^TM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cells, and HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for TBK1 at approximately 90 kDa (as indicated) using 20 µg/mL of Sheep Anti-Human TBK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF9934) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBK1

TANK binding kinase-1 (TBK1), encoded by the TBK1 gene, is a multimeric Serine/threonine-protein kinase that mediates a crosstalk between the innate immune response and autophagy. TBK1 was first identified as a TANK-interacting protein controlling NF-kappa B-mediated responses. Mutations in TBK1 have been found in conditions like amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), normal tension glaucoma (NTG) and childhood herpes simplex encephalitis (HSE). Gain-of-function of TBK1 are associated with NTG, whereas loss-of-function mutations result in ALS/FTD or in HSE.

Long Name

TANK Binding Kinase 1

Entrez Gene IDs

29110 (Human); 56480 (Mouse); 299827 (Rat)

Alternate Names

EC 2.7.11; EC 2.7.11.1; FLJ11330; FTDALS4; NAK serine/threonine-protein kinase TBK 1; NAK; NAKserine/threonine-protein kinase TBK1; NF-kappa-B-activating kinase; NFKB-Activating Kinase; T2K; TANK-binding kinase 1NF-kB-activating kinase; TBK 1; TBK1