Human TBX2 Antibody

Catalog #: MAB5040 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5040
MAB5040-SP

Detection of Human TBX2 by Western Blot.

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All TBX2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TBX2 Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human TBX2 in Western blots at approximately 75 kDa.

Source

Monoclonal Rat IgG_2B Clone # 393725

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TBX2
Thr593-Arg702
Accession # Q13207

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TBX2 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line, JEG-3 human epithelial choriocarcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line, and BG0IV human embryonic stem cells. PVDF membrane was probed with 0.1 µg/mL of Human TBX2 Monoclonal Antibody (Catalog # MAB5040) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TBX2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX2

TBX2 (T-box transcription factor 2) is a member of a family of transcription factors that share the T-box DNA binding domain. TBX2, as well as other TBX proteins, is involved in remodeling of the heart myocardium through a complex spatial and temporal regulation. TBX2 is associated with tumorigenesis and anti-senescence properties by affecting several cyclin-dependent kinase inhibitors. The full-length protein contains 702 amino acids with a DNA binding domain from aa 99 to aa 277.

Long Name

T-box Transcription Factor 2

Entrez Gene IDs

6909 (Human); 21385 (Mouse); 303398 (Rat)

Alternate Names

FLJ10169; T-box 2; T-box protein 2; T-box transcription factor TBX2; TBX2