Human TBX3 Antibody

Catalog #: AF4509
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4509
AF4509-SP

Detection of Human TBX3 by Western Blot.

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All TBX3 Antibodies

Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TBX3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TBX3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TBX2, rhTBX6, and rhTBX20 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TBX3
Ser321-Thr483
Accession # O15119

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1-2 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TBX3 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for TBX3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Western Blot

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Detection of Human TBX3 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for TBX3 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry

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TBX3 in LNCaP Human Cell Line. TBX3 was detected in immersion fixed LNCaP human prostate cancer cell line using Goat Anti-Human TBX3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4509) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX3

TBX3 belongs to the T-box family of transcription factors, which are involved in regulation of developmental processes. TBX3 acts as a transcriptional repressor regulating anterior/posterior axis of the forelimb. Mutations in TBX3 are associated with ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. TBX3 has 3 splice isoforms.

Long Name

T-box Transcription Factor 3

Entrez Gene IDs

6926 (Human); 21386 (Mouse); 353305 (Rat)

Alternate Names

bladder cancer related protein XHL; T-box 3; T-box protein 3; T-box transcription factor TBX3; TBX3; TBX3-ISO; ulnar mammary syndrome; UMS; XHL

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TBX3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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scRNA-sequencing reveals new enteric nervous system roles for GDNF, NRTN, and TBX3
Authors: CM Wright, S Schneider, KM Smith-Edwa, F Mafra, AJL Leembrugge, MV Gonzalez, DR Kothakapa, JB Anderson, BA Maguire, T Gao, TA Missall, MJ Howard, JC Bornstein, BM Davis, RO Heuckeroth
Cellular and Molecular Gastroenterology and Hepatology, 2021-01-11;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Loss of Tbx3 in murine neural crest reduces enteric glia and causes cleft palate, but does not influence heart development or bowel transit
Authors: Silvia Huerta López, Marina Avetisyan, Christina M. Wright, Karim Mesbah, Robert G. Kelly, Anne M. Moon et al.
Developmental Biology