Human TDRD1 Antibody

Catalog #: AF6296 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6296
AF6296-SP

Detection of Human TDRD1 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TDRD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TDRD1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse TDRD1 is observed and less than 1% cross-reactivity with recombinant human TDRKH is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human TDRD1
Asn837-Lys968
Accession # Q9BXT4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TDRD1 by Western Blot. Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human TDRD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6296) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for TDRD1 at approximately 75 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDRD1

TDRD1 (Tudor domain containing protein 1; also CT41.1) is a 120-140 kDa member of the Tudor family of proteins. It is expressed in spermatocytes and spermatids, and forms part of an RNA-silencing complex that regulates gene expression. TDRD1, through its Tudor and MYND domains, binds methylated PIWI (P-element-induced wimpy testis) protein and PIWI-interacting RNA loading factor, respectively. This serves to promote the recruitment of germline-specific small RNAs into a PIWI ribonucleoprotein complex. Human TDRD1 is 1180 aa in length. It contains one MYND-type Zn finger region (aa 170-206), and four methyl-binding Tudor domains (aa 312-1048). There are four potential splice variants with predicted molecular weights ranging between 79 kDa and 133 kDa. One contains an alternate start site at Met404, another contains an alternative start site at Met22, accompanied by deletions of aa 554-610 and 775-831, a third shows a 10 aa substitution for Ser1180, while a fourth contains deletions of aa 328-375, 554-610 and 1059-1134. Over aa 837-968, human TDRD1 shares 66% aa identity with mouse TDRD1.

Long Name

Tudor Domain Containing 1

Entrez Gene IDs

56165 (Human); 83561 (Mouse); 292129 (Rat)

Alternate Names

CT41.1; CT41.1Cancer/testis antigen 41.1; FLJ21082; TDRD1; tudor domain containing 1; tudor domain containing protein 1; tudor domain-containing protein 1