Human TDRKH Antibody

Name: Human TDRKH Antibody
Brand: R&D Systems
SKU: MAB6286
Rating: 5 (1 reviews)

Catalog #: MAB6286 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6286
MAB6286-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TDRKH Antibody Summary

Species Reactivity

Human

Specificity

Detects human TDRKH in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human TDRD1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 686213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human TDRKH
His206-Leu561
Accession # Q9Y2W6

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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TDRKH in Adult Mouse Testis. TDRKH was detected in immersion fixed frozen sections of adult mouse testis using Mouse Anti-Human TDRKH Monoclonal Antibody (Catalog # MAB6286) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of germ cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TDRKH

TDRKH (Tudor domain and KH domain containing protein; also TDRD2) is a 70 kDa member of the Tudor family of proteins. It is expressed in developing spermatogonia and meiotic primary spermatocytes, but not embryonic stem cells. TDRKH forms part of an RNA-silencing complex that safeguards the germ cell genome. Through its 60 amino acid (aa) Tudor domain, it binds methylated PIWI (P-element-induced wimpy testis) protein and serves to position PIWI for noncoding RNA binding. Human TDRKH is 606 aa in length. It contains two RNA recognition KH domains (aa 52-115 and 124-190), and one methyl-binding Tudor domain (aa 353-412). There are four potential splice forms. Three possess a shared deletion of aa 562-606, with two of these also showing a unique deletion of aa 108-152 and 76-79, respectively; a fourth shows a two aa substitution for aa 559-606. Over aa 206-561, human TDRKH shares 87% aa identity with mouse TDRKH.

Long Name

Tudor and KH Domain Containing

Entrez Gene IDs

11022 (Human); 72634 (Mouse); 310652 (Rat)

Alternate Names

TDRD2; TDRKH; tudor and KH domain containing; tudor and KH domain-containing protein