Human TEM5/GPR124 Antibody

Catalog #: MAB88961 Datasheet / COA / SDS

Human TEM5/GPR124 MAb (Clone 499708)

Catalog #	Availability	Size / Price	Qty
MAB88961-100
MAB88961-SP

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Detection of TEM5/GPR124 in CHO-S Transfected (positive) and CHO-S Wild Type (negative) Cells.

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All TEM5/GPR124 Antibodies

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Human TEM5/GPR124 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TEM5/GPR124 in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 499708

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human TEM5/GPR124
Met1-Leu699
Accession # Q96PE1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

3-25 µg/mL

Immersion fixed CHO-S transfected (positive) and CHO-S wild type (negative) cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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Detection of TEM5/GPR124 in CHO-S Transfected (positive) and CHO-S Wild Type (negative) Cells. TEM5/GPR124 was detected in immersion fixed CHO-S transfected (positive) and CHO-S wild type (negative) cells using Mouse Anti-Human TEM5/GPR124 Monoclonal Antibody (Catalog # MAB88961) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEM5/GPR124

G protein-coupled receptor 124 (GPR124, also known as TEM5), is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. GPR124 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. This receptor mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans. GPR124 has also been shown to interact with DLG1 through its PDZ-binding motif.

Long Name

Tumor Endothelial Marker 5

Entrez Gene IDs

25960 (Human); 78560 (Mouse)

Alternate Names

DKFZp434C211; DKFZp434J0911; G protein-coupled receptor 124; GPR124; G-protein coupled receptor 124; KIAA1531; KIAA1531FLJ14390; TEM5; TEM5Tumor endothelial marker 5