Human Testican 1/SPOCK1 Antibody

Catalog # Availability Size / Price Qty
MAB2327
MAB2327-SP
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews

Human Testican 1/SPOCK1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Testican 1/SPOCK1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Testican 2 or rhTestican 3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 316801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Testican 1/SPOCK1
Arg22-Trp439
Accession # Q08629
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Testican 1/SPOCK1 (Catalog # 2327-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 1/SPOCK1

Testican 1, also known as SPOCK1, was originally identified in seminal plasma and is also expressed in brain. It is a proteoglycan that contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites as well as three potential inhibitory domains targeted toward three different classes of proteases, metallo-, cysteine and serine proteases. Human Testican 1 shares 95% amino acid sequence identity with mouse Testican 1.

Entrez Gene IDs
6695 (Human)
Alternate Names
FLJ37170; Protein SPOCK; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1; SPOCK1; SPOCKcwcv and kazal-like domains proteoglycan (testican); Testican 1; TIC1; TICN1

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Citations for Human Testican 1/SPOCK1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. SPOCK1 is a novel inducer of epithelial to mesenchymal transition in drug-induced gingival overgrowth
    Authors: R Alshargabi, T Sano, A Yamashita, A Takano, T Sanada, M Iwashita, T Shinjo, T Fukuda, T Sanui, S Kishida, F Nishimura
    Sci Rep, 2020-06-17;10(1):9785.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin.
    Authors: Krishnatry AS, Brazeau DA, Fung HL
    Nitric Oxide, 2009-10-15;22(1):11-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot

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