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Human Testican 2/SPOCK2 Antibody

Catalog #: MAB2328
1 Citation Datasheet / COA / SDS
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MAB2328
MAB2328-SP
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Citations (1)
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Human Testican 2/SPOCK2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Testican 2/SPOCK2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Testican 1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 327403
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Testican 2/SPOCK2
Glu23-Trp424
Accession # Q92563
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Testican 2/SPOCK2 (Catalog # 2328-PI) under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Testican 2/SPOCK2 (Catalog # 2328-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Testican 2/SPOCK2

Testican 2 encoded by the SPOCK2 gene is a proteoglycan highly expressed in brain (1, 2). The human and mouse cDNAs predict 94% identity between the deduced amino acid sequences from the two species, indicating a conserved function (3, 4). Testican 2 contains a Ca2+-binding domain and a C-terminal acidic domain with putative glycosaminoglycan attachment sites. In addition, it contains three potential inhibitory domains targeted toward three different classes of proteases, metallo, cysteine and serine proteases. However, these domains may not be involved directly in the inhibition of targeted proteases. For example, Testican 2 is not able to inhibit MMP-14 (MT1-MMP, a metalloprotease) activation of MMP-2 (5). Nonetheless, overexpression of Testican 2 is able to abrogate inhibition of MMP-14 by other testican family members and to inhibit neurite outgrowth (6, 7).

References
  1. Nagase, T. et al. (1996) DNA Res. 3:321.
  2. Vannahme, C. et al. (1999) J. Neurochem. 73:12.
  3. Strausberg, R.L. et al. (2002) Proc. Natl. Acad. Sci. USA 99:16899.
  4. Okazaki, Y. et al. (2002) Nature 420:563.
  5. Nakada, M. et al. (2001) Cancer Res. 61:8896.
  6. Nakada, M. et al. (2003) Cancer Res. 63:3364.
  7. Hartmann, U. and P. Maurer (2001) Matrix Biol. 20:23.
Entrez Gene IDs
9806 (Human)
Alternate Names
FLJ97039; KIAA0275; KIAA0275CWCV, and Kazal-like domains proteoglycan 2; sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 2; SPOCK2; Testican 2

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Citation for Human Testican 2/SPOCK2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing
    Authors: A Basu, S Munir, MA Mulaw, K Singh, B Herold, D Crisan, A Sindrilaru, N Treiber, M Wlaschek, M Huber-Lang, F Gebhard, K Scharffett
    Sci Rep, 2018-04-18;8(1):6205.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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