Human TFPI-2 Antibody

Catalog #: MAB2545
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2545
MAB2545-SP

All TFPI-2 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFPI-2 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2A Clone # 243220

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI-2
Asp23-Phe235
Accession # P48307

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human TFPI‑2 (Catalog # 2545-PI) under non-reducing conditions only

Immunohistochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded sections of human placenta

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human TFPI‑2 (Catalog # 2545‑PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI-2

Human Tissue Factor Pathway Inhibitor 2 (TFPI-2), also known as placental protein 5 (PP5) and retinal pigment epithelial cell factor 1 (REF-1), is a secreted protein with an N-terminal acidic region, three Kunitz (K) domains (amino acids 36 to 86, 96 to 149 and 158 to 208) separated with by two linker regions, and a C-terminal basic region (1‑3). It is a Kunitz-type serine protease inhibitor secreted by all cells of the vasculature (endothelial cells, smooth muscle cells, and fibroblasts) into the extracellular matrix (ECM), where it plays a role in the regulation of urokinase/plasmin-mediated ECM turnover. Expression of TFPI-2 is down-regulated in several cancers, which may contribute to tumor progression in these cancers (4).

References

Miyagi, Y. et al. (1994) J. Biochem. 116:939.
Sprecher, C.A. et al. (1994) Proc. Natl. Acad. Sci. USA 91:3353.
Tanaka, Y. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45:245.
Rollin, J. et al. (2005) Br, J. Cancer 92:775.

Long Name

Tissue Factor Pathway Inhibitor 2

Entrez Gene IDs

7980 (Human)

Alternate Names

Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI2; TFPI-2; TFPI-2REF1; tissue factor pathway inhibitor 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TFPI-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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