Do the TFPI antibodies (Catalog #s MAB2974, MAB29741, and BAF2974) detect TFPI-alpha or TFPI-beta?

Name: Human TFPI Antibody
Brand: R&D Systems
SKU: MAB29741
Price: 559 USD

These antibodies were raised against Asp29-Lys282 of isoform 1 of Accession # P10646. Isoform 1 is also known as TFPI-alpha. We do not epitope map our antibodies, so we do not know where within Asp29-Lys282 these antibodies bind. Since TFPI-alpha and TFPI-beta share 100% homology from aa1-209, it is possible that our antibodies recognize both the alpha and beta forms of TFPI.

Human TFPI Antibody

Catalog #: MAB29741 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29741
MAB29741-SP

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFPI in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Coagulation factor II, VII, XI, XIV, rhTFPI-2, recombinant mouse (rm) TFPI, rmTFPI-2, or rhVitronectin is observed.

Source

Monoclonal Mouse IgG_2B Clone # 374718

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI
Asp29-Lys282
Accession # P10646

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Human TFPI Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human TFPI Biotinylated Antibody (Catalog # BAF2974)

Standard: Recombinant Human TFPI Protein, CF (Catalog # 2974-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of rhCoagulation Factor VII (R&D Systems, Catalog # 2338-SE) in the presence of rhTissue Factor (R&D Systems, Catalog # 2339-PA). Human TFPI shares 60%, 59% and 58% amino acid sequence identity with rat, mouse and canine TFPI, respectively.

References

Bai, H. et al. (2005) Thromb Haemost. 93:1055.
Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
Mine, S. et al. (2002) Biochemistry 41:78.

Long Name

Tissue Factor Pathway Inhibitor

Entrez Gene IDs

7035 (Human); 21788 (Mouse)

Alternate Names

convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

Do the TFPI antibodies (Catalog #s MAB2974, MAB29741, and BAF2974) detect TFPI-alpha or TFPI-beta?
- These antibodies were raised against Asp29-Lys282 of isoform 1 of Accession # P10646. Isoform 1 is also known as TFPI-alpha. We do not epitope map our antibodies, so we do not know where within Asp29-Lys282 these antibodies bind. Since TFPI-alpha and TFPI-beta share 100% homology from aa1-209, it is possible that our antibodies recognize both the alpha and beta forms of TFPI.

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