Human TFPI Antibody

Catalog #: AF2974
8 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2974
AF2974-SP

Detection of Human TFPI by Western Blot.

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All TFPI Antibodies

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Citations (8)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFPI in direct ELISAs and Western blots. In directs ELISAs, less than 10% cross‑reactivity with recombinant mouse (rm) TFPI is observed and less than 1% cross-reactivity with recombinant human TFPI-2, and rmTFPI-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI
Asp29-Lys282
Accession # P10646

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

50 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human TFPI (Catalog # 2974-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human TFPI by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFPI at approximately 40-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human TFPI by Simple Western^TM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for TFPI at approximately 58 kDa (as indicated) using 50 µg/mL of Goat Anti-Human TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2974) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA).

References

Bai, H. et al. (2005) Thromb Haemost. 93:1055.
Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
Mine, S. et al. (2002) Biochemistry 41:78.

Long Name

Tissue Factor Pathway Inhibitor

Entrez Gene IDs

7035 (Human); 21788 (Mouse)

Alternate Names

convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human TFPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist-induced platelet functions reducing atherosclerosis and thrombosis phenotypes
Authors: A Al Subayyi, YS Basmaeil, R Alenzi, T Khatlani
Journal of Cellular and Molecular Medicine, 2021-09-18;0(0):.
TMEM16E regulates endothelial cell procoagulant activity and thrombosis
Authors: Alec A. Schmaier, Papa F. Anderson, Siyu M. Chen, Emale El-Darzi, Ivan Aivasovsky, Milan P. Kaushik et al.
Journal of Clinical Investigation
Aberrant stromal tissue factor localisation and mycolactone-driven vascular dysfunction, exacerbated by IL-1beta, are linked to fibrin formation in Buruli ulcer lesions
Authors: LT Hsieh, SJ Dos Santos, BS Hall, J Ogbechi, AD Loglo, FJ Salguero, MT Ruf, G Pluschke, RE Simmonds
PloS Pathogens, 2022-01-31;18(1):e1010280.
Species: Human
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: ELISA Detection, Western Blot
The Impact of Anticoagulant Activity of Tissue Factor Pathway Inhibitor Measured by a Novel Functional Assay for Predicting Deep Venous Thrombosis in Trauma Patients: A Prospective Nested Case-Control Study
Authors: Xiangyu Cao, Yu Su, Wenjie Zhang, Huiru Zhao, Meng Wen, Shan Lu et al.
Clin Appl Thromb Hemost
SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells
Authors: CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi
Cell Rep, 2019-09-24;28(13):3329-3337.e5.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
Enzymatically oxidized phospholipids restore thrombin generation in coagulation factor deficiencies
Authors: DA Slatter, CL Percy, K Allen-Redp, JM Gajsiewicz, NJ Brooks, A Clayton, VJ Tyrrell, M Rosas, SN Lauder, A Watson, M Dul, Y Garcia-Dia, M Aldrovandi, M Heurich, J Hall, JH Morrissey, S Lacroix-De, S Delignat, PV Jenkins, PW Collins, VB O'Donnell
JCI Insight, 2018-03-22;3(6):.
Species: Human
Sample Types: Plasma
Applications: Neutralization
Correcting thrombin generation ex vivo using different haemostatic agents following cardiac surgery requiring the use of cardiopulmonary bypass
Authors: Charles L. Percy, Rudolf Hartmann, Rhidian M. Jones, Subramaniam Balachandran, Dheeraj Mehta, Michael Dockal et al.
Blood Coagulation & Fibrinolysis
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Authors: Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
Mol. Cell Proteomics, 2008-04-03;7(10):1974-82.
Species: Human
Sample Types: Serum
Applications: Mass Spectrometry