Do the TFPI antibodies (Catalog #s MAB2974, MAB29741, and BAF2974) detect TFPI-alpha or TFPI-beta?

Name: Human TFPI Biotinylated Antibody
Brand: R&D Systems
SKU: BAF2974
Price: 539 USD

These antibodies were raised against Asp29-Lys282 of isoform 1 of Accession # P10646. Isoform 1 is also known as TFPI-alpha. We do not epitope map our antibodies, so we do not know where within Asp29-Lys282 these antibodies bind. Since TFPI-alpha and TFPI-beta share 100% homology from aa1-209, it is possible that our antibodies recognize both the alpha and beta forms of TFPI.

Human TFPI Biotinylated Antibody

Catalog #: BAF2974
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2974

All TFPI Antibodies

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TFPI Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human TFPI in ELISAs and Western blots. In sandwich immunoassays, less than 0.04% cross-reactivity with recombinant mouse (rm) TFPI, recombinant human TFPI-2, and rmTFPI-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human TFPI
Asp29-Lys282
Accession # P10646

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human TFPI (Catalog # 2974-PI)

Human TFPI Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human TFPI Antibody (Catalog # MAB29741)

Standard: Recombinant Human TFPI Protein, CF (Catalog # 2974-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI

Human TFPI, also known as lipoprotein-associated coagulation inhibitor (LACI) and extrinsic pathway inhibitor (EPI), is a physiological inhibitor of extrinsic pathway of coagulation and has biological functions of anticoagulation and anti-inflammation (1). It is a secreted protein with a N-terminal acidic region, three Kunitz (K) domains separated with by two linker regions, and a C-terminal basic region (2). The first K domain (residues 54 to 104) inhibits coagulation factor VIIa complexed to tissue factor (TF). The second K domain (residues 125 to 175) inhibits factor Xa. The third K domain (residues 217 to 267) binds to heparin (3). The C-terminal basic region may have several functions. For example, it plays an important role in binding of TFPI to cell surfaces (2). The purified rhTFPI ends at residue 282 and does not contain the last 20 residues (residues 283 to 302) in the C-terminal region. It inhibits the activity of Recombinant Human Coagulation Factor VII (Catalog # 2338-SE) in the presence of Recombinant Human Coagulation Factor III/Tissue Factor (Catalog # 2339-PA).

References

Bai, H. et al. (2005) Thromb Haemost. 93:1055.
Bajaj, M.S. et al. (2001) Thromb Haemost. 86:959.
Mine, S. et al. (2002) Biochemistry 41:78.

Long Name

Tissue Factor Pathway Inhibitor

Entrez Gene IDs

7035 (Human); 21788 (Mouse)

Alternate Names

convertin; EPI; EPITFPI1LACITFI; Extrinsic pathway inhibitor; LACI; Lipoprotein-associated coagulation inhibitor; TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); tissue factor pathway inhibitor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human TFPI Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Mechanistic Modeling of the Pharmacodynamic and Pharmacokinetic Relationship of Tissue Factor Pathway Inhibitor-Neutralizing Antibody (BAY 1093884) in Cynomolgus Monkeys
Authors: JM Gu, XY Zhao, T Schwarz, J Schuhmache, A Baumann, E Ho, B Subramanya, K Tran, T Myles, C Patel, M Koellnberg
AAPS J, 2017-05-17;0(0):.

FAQs

Do the TFPI antibodies (Catalog #s MAB2974, MAB29741, and BAF2974) detect TFPI-alpha or TFPI-beta?
- These antibodies were raised against Asp29-Lys282 of isoform 1 of Accession # P10646. Isoform 1 is also known as TFPI-alpha. We do not epitope map our antibodies, so we do not know where within Asp29-Lys282 these antibodies bind. Since TFPI-alpha and TFPI-beta share 100% homology from aa1-209, it is possible that our antibodies recognize both the alpha and beta forms of TFPI.

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