Human TGIF1 Antibody Summary
Lys245-Ser359
Accession # Q15583
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TGIF1 by Western Blot. Western blot shows lysates of HCT-116 human colorectal carcinoma cell line, JAR human choriocarcinoma cell line, and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human TGIF1 Monoclonal Antibody (Catalog # MAB7555) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TGIF1 at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human TGIF1 by Simple WesternTM. Simple Western lane view shows lysates of HCT‑116 human colorectal carcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for TGIF1 at approximately 38 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human TGIF1 Monoclonal Antibody (Catalog # MAB7555). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGIF1
TGIF1 (5'-TG[TCA]-3' Interacting Protein 1; also Transforming growth factor beta-induced factor and HPE4) is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa identity with mouse TGIF1.
Product Datasheets
Citation for Human TGIF1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Ghrelin alleviates 6-hydroxydopamine-induced neurotoxicity in SH-SY5Y cells
Authors: X He, W Yuan, CQ Yang, L Zhu, F Liu, J Feng, YX Xue
Neural regeneration research, 2022-01-01;17(1):170-177.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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