Human Th1/Th2 Luminex Performance Assay 11-plex Fixed Panel
Discontinued Product
LKTM008 has been discontinued and is replaced by LKTM008B.
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Human Th1/Th2 Luminex Performance Assay 11-plex Fixed Panel Summary
| Assay Type | bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and antibody-coated beads |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. |
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Precision
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Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays. |
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| n=40 (20x2 levels) | n=25 | |
|---|---|---|
| Analyte | Intra-Assay (%CV) | Inter-Assay (%CV) |
| GM-CSF | 3.1% | 10.3% |
| IFN-gamma | 2.8% | 10.4% |
| IL-10 | 3.3% | 9.0% |
| IL-12 p70 | 2.3% | 10.5% |
| IL-13 | 1.6% | 9.7% |
| IL-1beta | 2.3% | 10.0% |
| IL-2 | 2.2% | 9.2% |
| IL-4 | 3.2% | 9.7% |
| IL-5 | 2.2% | 12.0% |
| IL-6 | 2.6% | 9.8% |
| TNF-alpha | 2.2% | 8.7% |
Accuracy
|
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an astrisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples. |
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| Linearity | Spiked Recovery |
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|---|---|---|---|---|
| Analyte | N | Average % | N | Average % |
|
*Natural linearity |
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| GM-CSF | 4 | 96% | 4 | 110% |
| IFN-gamma | 4 | 97% | 4 | 112% |
| IL-10 | 4 | 111% | 4 | 104% |
| IL-12 p70 | 4 | 106% | 4 | 108% |
| IL-13 | 4 | 112% | 4 | 103% |
| IL-1beta | 4 | 107% | 4 | 99% |
| IL-2 | 4 | 105% | 4 | 102% |
| IL-4 | 3 | 127% | 3 | 77% |
| IL-5 | 3 | 113% | 3 | 81% |
| IL-6 | 3 | 124% | 3 | 90% |
| TNF-alpha | 4 | 117% | 4 | 69% |
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Scientific Data
Background: Th1/Th2 Assay Kits
T helper cells (Th) play a vital role in the adaptive immune system by suppressing or regulating immune responses. After activation, T helper cells differentiate into T-helper (Th) 1 or Th2 effector cells. These two subsets are characterized by their distinct cytokine expression pattern and the immune function they mediate. Th1/Th2 paradigm provides rationale for developing new vaccines against infectious diseases and novel strategies for the therapy of allergic and autoimmune disorders.
Th1 and Th2 cells each produce a specialized combination of cytokines that depends on type of pathogen that the helper T cell has recognized—some cytokines are more effective than others in the process of eliminating certain invaders
Product Datasheets
Citations for Human Th1/Th2 Luminex Performance Assay 11-plex Fixed Panel
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Safety and infectivity of female cercariae in Schistosoma-naïve, healthy participants: a controlled human Schistosoma mansoni infection study
Authors: Koopman, JPR;Houlder, EL;Janse, JJ;Casacuberta-Partal, M;Lamers, OAC;Sijtsma, JC;de Dood, C;Hilt, ST;Ozir-Fazalalikhan, A;Kuiper, VP;Roozen, GVT;de Bes-Roeleveld, LM;Kruize, YCM;Wammes, LJ;Smits, HH;van Lieshout, L;van Dam, GJ;van Amerongen-Westra, IM;Meij, P;Corstjens, PLAM;Jochems, SP;van Diepen, A;Yazdanbakhsh, M;Hokke, CH;Roestenberg, M;
EBioMedicine
Species: Human
Sample Types: Cell Culture Supernates
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T helper profile in pregnant women recovered from COVID-19
Authors: E Tartaglia, V Bordoni, A Oliva, A Vergori, E Girardi, A Antinori, C Agrati, G Liuzzi
Journal of reproductive immunology, 2022-07-06;153(0):103661.
Species: Human
Sample Types: Plasma
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Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for beta-Thalassemia Major Transfusion Therapy
Authors: D Schiroli, L Merolle, E Quartieri, R Chicchi, T Fasano, T De Luca, G Molinari, S Pulcini, TA Pertinhez, E Di Bartolo, R Biguzzi, R Baricchi, C Marraccini
Biomolecules, 2021-11-04;11(11):.
Species: Human
Sample Types: Cell Culture Supernates
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A Model Based on the Combination of IFN-gamma, IP-10, Ferritin and 25-Hydroxyvitamin D for Discriminating Latent From Active Tuberculosis in Children
Authors: P Comella-De, R Abellana, R Villar-Her, MD Jean Coute, B Sallés Min, L Canales Al, M Narcisse, J Gautier, C Ascaso, I Latorre, J Dominguez, TM Perez-Porc
Front Microbiol, 2019-08-14;10(0):1855.
Species: Human
Sample Types: Plasma
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