Human Themis Antibody

Catalog #: MAB6929 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6929
MAB6929-SP

Detection of Themis in Human Blood Lymphocytes by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Themis Antibody Summary

Species Reactivity

Human

Specificity

Detects human Themis in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Themis is observed.

Source

Monoclonal Mouse IgG_2A Clone # 701010

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Themis
Ala2-Pro282
Accession # Q8N1K5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Themis antibody in Human Blood Lymphocytes antibody by Flow Cytometry

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Detection of Themis in Human Blood Lymphocytes by Flow Cytometry Human peripheral blood lymphocytes were stained with Mouse Anti-Human Themis Monoclonal Antibody (Catalog # MAB6929) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # MAB003).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Themis

THEMIS (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, human THEMIS shares 86% aa sequence identity with mouse and rat THEMIS.

Long Name

Thymocyte-expressed Molecule Involved in Selection

Entrez Gene IDs

387357 (Human); 210757 (Mouse)

Alternate Names

bA325O24.3; bA325O24.4; C6orf190; C6orf207; FLJ40584; Gasp; GRB2-associated protein; MGC163388; protein THEMIS; signaling phosphoprotein specific for T cells; SPOT; Themis; thymocyte selection associated; thymocyte selection pathway associated; thymocyte-expressed molecule involved in selection; TSEPA