Human Tie-2 Antibody

Catalog #: MAB313
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB313
MAB313-SP

All Tie-2 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Tie-2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Tie-2 in direct ELISAs and Western blots. Shows approximately 15% cross-reactivity with recombinant mouse Tie‑2 and less than 1% cross-reactivity with recombinant human Tie-1.

Source

Monoclonal Mouse IgG₁ Clone # 83711

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Tie‑2
Ala23-Lys745
Accession # AAA61139

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Tie‑2 Fc Chimera (Catalog # 313-TI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tie-2

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.

Human Tie-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2), which bind Tie-2 with high-affinity have been identified. Ang-2 has been reported to act as an antagonist for Ang-1. Mice engineered to overexpress Ang-2 or to lack Ang-1 or Tie-2 display similar angiogenesis defects.

References

Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
Takakura, N. et al. (1998) Immunity 9:677.
Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2

Entrez Gene IDs

7010 (Human); 21687 (Mouse); 89804 (Rat); 396729 (Porcine); 403714 (Canine); 102122204 (Cynomolgus Monkey); 30747 (Zebrafish)

Alternate Names

angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Tie-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Acidic pH reduces VEGF-mediated endothelial cell responses by downregulation of VEGFR-2; relevance for anti-angiogenic therapies
Authors: Seraina Faes, Emilie Uldry, Anne Planche, Tania Santoro, Catherine Pythoud, Nicolas Demartines et al.
Oncotarget
Down-regulation of vascular endothelial growth factor receptor 2 is a major molecular determinant of proteasome inhibitor-mediated antiangiogenic action in endothelial cells.
Authors: Meissner M, Reichenbach G, Stein M, Hrgovic I, Kaufmann R, Gille J
Cancer Res., 2009-02-17;69(5):1976-84.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Monoclonal antibody classification based on epitope-binding using differential antigen disruption.
Authors: Shi E, Fury W, Li W, Mikulka W, Aldrich T, Rafique A, Chen G, Hoffenberg S, Daly TJ, Radziejewski C
J. Immunol. Methods, 2006-06-27;314(1):9-20.
Species: Human
Sample Types: Recombinant Protein
Applications: Biacore Binding Assay, Epitope Mapping
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Authors: Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood, 2003-03-20;102(2):638-45.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot