Human TIM-1/KIM-1/HAVCR Antibody
Human TIM-1/KIM-1/HAVCR Antibody Summary
Ser21-Thr288
Accession # AAC39862
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human TIM‑1/KIM‑1/HAVCR by Western Blot. Western blot shows lysates of human CD4+cells treated (+) with 5 µg/mL of Hamster Anti-Mouse CD3e Monoclonal Antibody (MAB484) and 1 µg/mL of Rat Anti-Mouse CD28 Monoclonal Antibody (MAB4831) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for TIM-1/KIM-1/HAVCR at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of TIM‑1/KIM‑1/HAVCR in Th2-stimulated Human PBMCs by Flow Cytometry. (A) Unstimulated and (B) Th2-stimulated human PBMCs were stained with Mouse Anti-Human TIM-1/KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B) and Human CD4 PerCP-conjugated Monoclonal Antibody (FAB3791C). Quadrant markers were set based on control antibody staining (Catalog # MAB0041).
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TIM‑1/KIM‑1/HAVCR in Human Kidney. TIM-1/KIM-1/HAVCR was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human TIM-1/ KIM-1/HAVCR Monoclonal Antibody (Catalog # MAB1750) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of TIM‑1/KIM‑1/HAVCR in Huh‑7 human hepatoma cell line by Flow Cytometry Huh‑7 human hepatoma cell line were stained with Mouse Anti-Human TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1750, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell-immunoglobulin-mucin; also KIM-1 and HAVcr-1) is a 100 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-3). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. Human TIM-1 is synthesized as a 359 amino acid (aa) precursor that contains a 20 aa signal sequence, a 270 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 48 aa cytoplasmic domain (4-6). The ECD contains oneV-type Ig-like domain and a mucin region characterized by multiple PTTTTL motifs. The mucin region undergoes extensive O-linked glycosylation. The TIM-1 gene is highly polymorphic and undergoes alternate splicing (1). For instance, the presence of a six aa sequence (MTTTVP) at position #137 of the mature molecule is associated with protection from atopy in people with a history of hepatitis A (7, 8). There are two cytoplasmic alternate splice forms of
TIM‑1. One is a long (359 aa) kidney form termed TIM-1b, and one is a short (334 aa) liver form termed TIM-1a. Both are identical through the first 323 aa of their precursors. TIM-1b contains a tyrosine phosphorylation motif that is not present in 1a (6). TIM-1 is also known to circulate as a soluble form. Constitutive cleavage by an undefined MMP (possibly ADAM33) releases an 85 - 90 kDa soluble molecule (6). The ECD of human TIM-1 is 50% and 43% aa identical to mouse and canine TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (4, 9). However, others are believed to exist, and based on the ligand for TIM-3, one may well be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10).
- Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471.
- Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454.
- Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
- Feigelstock, D. et al. (1998) J. Virol. 72:6621.
- Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.
- Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
- Umetsu, D.T. et al. (2005) J. Pediatr. Gastroenterol. Nutr. 40:S43.
- Gao, P-S. et al. (2005) J. Allergy Clin. Immunol. 115:982.
- Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
- Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Product Datasheets
Product Specific Notices
This product is covered by one or more of the following US Patents 7,300,652; 7,041,290; 6,664,385 and other US and foreign patents pending or issued.Citations for Human TIM-1/KIM-1/HAVCR Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Microphysiological Systems to Assess Nonclinical Toxicity
Authors: Kirk P. Van Ness, Shih‐Yu Chang, Elijah J. Weber, Danielle Zumpano, David L. Eaton, Edward J. Kelly
Current Protocols in Toxicology
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Interaction between hTIM-1 and Envelope Protein Is Important for JEV Infection
Authors: Liang, Z;Pan, J;Xie, S;Yang, X;Cao, R;
Viruses
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TIM-1 promotes proliferation and metastasis, and inhibits apoptosis, in cervical cancer through the PI3K/AKT/p53 pathway
Authors: L Chen, J Qing, Y Xiao, X Huang, Y Chi, Z Chen
BMC Cancer, 2022-04-07;22(1):370.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Identification of the SHREK family of proteins as broad-spectrum host antiviral factors
Authors: D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu
bioRxiv : the preprint server for biology, 2021-02-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Dynamic Dystroglycan Complexes Mediate Cell Entry of Lassa Virus
Authors: A Herrador, C Fedeli, E Radulovic, KP Campbell, H Moreno, G Gerold, S Kunz
MBio, 2019-03-26;10(2):.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
TIM-1 Promotes Japanese Encephalitis Virus Entry and Infection
Authors: J Niu, Y Jiang, H Xu, C Zhao, G Zhou, P Chen, R Cao
Viruses, 2018-11-14;10(11):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
Physiol Rep, 2018-03-01;6(6):e13654.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Ebola Virus Binding to Tim-1 on T Lymphocytes Induces a Cytokine Storm.
Authors: Patrick Younan, Mathieu Iampietro, Andrew Nishida, Palaniappan Ramanatha, Rodrigo I Santos, Mukta Dutta, Ndongala Michel Lubaki, Richard A Koup, Michael G Katze, Alexander Bukreyev
MBio, 2017-09-26;0(0):2150-7511.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
TIM1 (HAVCR1) Is Not Essential for Cellular Entry of Either Quasi-enveloped or Naked Hepatitis A Virions
Authors: A Das, A Hirai-Yuki, O González-L, B Rhein, S Moller-Tan, R Brouillett, L Hensley, I Misumi, W Lovell, JM Cullen, JK Whitmire, W Maury, SM Lemon
MBio, 2017-09-05;8(5):.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot -
Attachment and Post-Attachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission
Authors: H Fan, L Qiao, KD Kang, J Fan, W Wei, G Luo
J. Virol., 2017-06-09;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Primary Human Placental Trophoblasts are Permissive for Zika Virus (ZIKV) Replication
Authors: KM Aagaard, A Lahon, MA Suter, RP Arya, MD Seferovic, MB Vogt, M Hu, F Stossi, MA Mancini, RA Harris, M Kahr, C Eppes, M Rac, MA Belfort, CS Park, D Lacorazza, R Rico-Hesse
Sci Rep, 2017-01-27;7(0):41389.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
TIM-1 promotes hepatitis C virus cell attachment and infection
Authors: Guangxiang Luo
J. Virol., 2017-01-03;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of Tim-1 in primary CNS lymphoma
Cancer Med, 2016-10-05;5(11):3235-3245.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A polymorphism of the TIM-1 IgV domain: implications for the susceptibility to filovirus infection.
Authors: Kuroda M, Fujikura D, Noyori O, Kajihara M, Maruyama J, Miyamoto H, Yoshida R, Takada A
Biochem Biophys Res Commun, 2014-11-04;455(3):223-8.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Acute pretreatment with chloroquine attenuates renal I/R injury in rats.
Authors: Todorovic, Zoran, Medic, Branisla, Basta-Jovanovic, Gordana, Radojevic Skodric, Sanja, Stojanovic, Radan, Rovcanin, Branisla, Prostran, Milica
PLoS ONE, 2014-03-28;9(3):e92673.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Laser capture microdissection and multiplex-tandem PCR analysis of proximal tubular epithelial cell signaling in human kidney disease.
Authors: Wilkinson R, Wang X, Kassianos A, Zuryn S, Roper K, Osborne A, Sampangi S, Francis L, Raghunath V, Healy H
PLoS ONE, 2014-01-27;9(1):e87345.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
TIM-family proteins promote infection of multiple enveloped viruses through virion-associated phosphatidylserine.
Authors: Jemielity, Stephani, Wang, Jinyize, Chan, Ying Kai, Ahmed, Asim A, Li, Wenhui, Monahan, Sheena, Bu, Xia, Farzan, Michael, Freeman, Gordon J, Umetsu, Dale T, Dekruyff, Rosemari, Choe, Hyeryun
PLoS Pathog, 2013-03-28;9(3):e1003232.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Gene-trap mutagenesis identifies mammalian genes contributing to intoxication by Clostridium perfringens epsilon-toxin.
Authors: Ivie SE, Fennessey CM, Sheng J, Rubin DH, McClain MS
PLoS ONE, 2011-03-11;6(3):e17787.
Species: Human
Sample Types: Whole Cells
Applications: Cell-based ELISA, ICC -
Altered expression of T cell immunoglobulin-mucin (TIM) molecules in bronchoalveolar lavage CD4+ T cells in sarcoidosis.
Authors: Idali F, Wahlstrom J, Dahlberg B, Khademi M, Olsson T, Eklund A, Grunewald J
Respir Res, 2009-05-29;10(0):42.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Kidney injury molecule‑1 levels are associated with therapeutic outcomes and renal tubulointerstitial injury severity in idiopathic membranous nephropathy
Authors: Yidan Zhang, Chunhong Xiang, Liying Gong, Yuanyuan Zhang, Junhui Zhen, Zhao Hu et al.
Experimental and Therapeutic Medicine
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Naringenin Alleviates Renal Ischemia Reperfusion Injury by Suppressing ER Stress-Induced Pyroptosis and Apoptosis through Activating Nrf2/HO-1 Signaling Pathway
Authors: Banghua Zhang, Shanshan Wan, Hao Liu, Qiangmin Qiu, Hui Chen, Zhiyuan Chen et al.
Oxidative Medicine and Cellular Longevity
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