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Human TIM-4 Antibody

Catalog #: AF2929
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2929
AF2929-SP
Detection of Recombinant Human TIM‑4 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TIM-4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human TIM-4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) TIM-1 and recombinant mouse (rm) TIM-4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIM-4 (R&D Systems, Catalog # 2929-TM)
Glu25-Leu315
Accession # Q96H15
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human TIM-4 antibody by Western Blot. View Larger

Detection of Recombinant Human TIM‑4 by Western Blot. Western blot shows 25 ng of Recombinant Human TIM-4 His-tag (Catalog # 2929-TM), Recombinant Human TIM-1/KIM-1/HAVCR (Catalog # 1750-TM), Recombinant Human TIM-3 Fc Chimera (Catalog # 2365-TM), Recombinant Mouse TIM-1/KIM-1/HAVCR (Catalog # 1817-TM), Recombinant Mouse TIM-2 (Catalog # 1885-TM), Recombinant Mouse TIM-3 Fc Chimera (Catalog # 1529-TM), Recombinant Mouse TIM-4 (Catalog # 2826-TI), Recombinant Mouse TIM-5, and Recombinant Mouse TIM-6. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human TIM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2929) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TIM-4 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry TIM-4 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

TIM‑4 in Human Tonsil. TIM-4 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human TIM-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2929) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-4

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4+ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

References
  1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. 
  3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
  4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
  5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
  6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  8. Khademi, M. et al. (2004) J. Immunol. 172:7169.
Long Name
T Cell Immunoglobulin Mucin-4
Entrez Gene IDs
91937 (Human); 276891 (Mouse); 102140672 (Cynomolgus Monkey)
Alternate Names
FLJ27515; Smuckler; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell membrane protein 4; TIM4; TIM-4; TIMD4; TIMD-4

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Citations for Human TIM-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Human iPSC-Derived Cardiomyocytes Are Susceptible to SARS-CoV-2 Infection
    Authors: Arun Sharma, Gustavo Garcia, Yizhou Wang, Jasmine T. Plummer, Kouki Morizono, Vaithilingaraja Arumugaswami et al.
    Cell Reports Medicine
  2. Blocking elevated p38 MAPK restores efferocytosis and inflammatory resolution in the elderly
    Authors: Roel P. H. De Maeyer, Rachel C. van de Merwe, Rikah Louie, Olivia V. Bracken, Oliver P. Devine, Daniel R. Goldstein et al.
    Nature Immunology
  3. Phagocytosing differentiated cell-fragments is a novel mechanism for controlling somatic stem cell differentiation within a short time frame
    Authors: Wakao S, Oguma Y, Kushida Y et al.
    Research Square
  4. Immortalized stem cell-derived hepatocyte-like cells: An alternative model for studying dengue pathogenesis and therapy
    Authors: K Kongmanas, N Punyadee, K Wasuworawo, A Songjaeng, T Prommool, Y Pewkliang, S Manocheewa, S Thiemmeca, K Sa-Ngiamsu, C Puttikhunt, KF Faull, S Hongeng, P Avirutnan
    PLoS Negl Trop Dis, 2020-11-20;14(11):e0008835.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Differentiation Paths of Peyer's Patch LysoDCs Are Linked to Sampling Site Positioning, Migration, and T Cell Priming
    Authors: C Wagner, J Bonnardel, C Da Silva, L Spinelli, CA Portilla, J Tomas, M Lagier, L Chasson, M Masse, M Dalod, A Chollat-Na, JP Gorvel, H Lelouard
    Cell Rep, 2020-04-07;31(1):107479.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Human Sertoli cells support high levels of Zika virus replication and persistence
    Authors: A Kumar, J Jovel, J Lopez-Oroz, D Limonta, AM Airo, S Hou, I Stryapunin, C Fibke, RB Moore, TC Hobman
    Sci Rep, 2018-04-03;8(1):5477.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Characterizing functional domains for TIM-mediated enveloped virus entry.
    Authors: Moller-Tank S, Albritton L, Rennert P, Maury W
    J Virol, 2014-04-02;88(12):6702-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells.
    Authors: Kobayashi N, Karisola P, Pena-Cruz V, Dorfman DM, Jinushi M, Umetsu SE, Butte MJ, Nagumo H, Chernova I, Zhu B, Sharpe AH, Ito S, Dranoff G, Kaplan GG, Casasnovas JM, Umetsu DT, DeKruyff RH, Freeman GJ
    Immunity, 2007-12-01;27(6):927-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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