Human TM4SF4 PE-conjugated Antibody

Catalog #: FAB7998P Datasheet / COA / SDS

Discontinued Product

FAB7998P has been discontinued.
View all TM4SF4 products.
Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry.
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Human TM4SF4 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human TM4SF4 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 832441
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human TM4SF4
Accession # P48230
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TM4SF4 antibody in HT‑29 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of TM4SF4 in HT‑29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human TM4SF4 PE-conjugated Monoclonal Antibody (Catalog # FAB7998P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TM4SF4

TM4SF4 (Transmembrane 4 L6 Family Member 4), also known as IL-TMP (Intestine and Liver Tetraspanin Membrane Protein), is a distant member of the tetraspanin L6 domain family of molecules. It is expressed at highest levels on non-proliferating villus-associated intestinal epithelia of the jejunum, periportal hepatocytes, and follicle-associated epithelium of the ileum. It is thought to mediate proliferation and adhesion, and is now known to complex with THTR-2 (Thiamine Transporter-2) during the transport of thiamine into expressing cells. Its molecular size is reported at approximately 21 kDa for the unglycosylated form and varies from 25-30 kDa on proliferating cells, to 25-40 kDa on non-proliferating cells.  The 202 amino acid (aa) human TM4SF4 contains four transmembrane domains and two potential N-glycosylation sites. The extracellular region of human TM4SF4 shares 72% aa sequence identity with mouse TM4SF4.

Long Name
Transmembrane 4 L Six Family Member 4
Entrez Gene IDs
7104 (Human); 229302 (Mouse); 116467 (Rat)
Alternate Names
FLJ31015; ILTMP; il-TMP; ILTMPintestinal and liver (il) tetraspan membrane protein; Intestine and liver tetraspan membrane protein; TM4SF4; transmembrane 4 L six family member 4; transmembrane 4 L6 family member 4; transmembrane 4 superfamily member 4

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