Human TMED1 Antibody

Catalog #: MAB2243 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2243-100
MAB2243-SP

Detection of TMED-1 in HEK293 Human Cell Line Transfected with Human TMED-1 and eGFP by Flow Cytometry.

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All TMED1 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human TMED1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human TMED1 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 1009527

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human embryonic kidney cell, HEK293 derived human TMED1
Met1-Asn194
Accession # Q13445

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TMED-1 in HEK293 Human Cell Line Transfected with Human TMED-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) TMED-1 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human TMED-1 Monoclonal Antibody (Catalog # MAB2243) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG1 Isotype Control Antibody staining (Catalog # MAB002, data not shown). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry

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TMED1 in A549 Human Cell Line. TMED1 was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human TMED1 Monoclonal Antibody (Catalog # MAB2243) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TMED1

TMED1 (Transmembrane Emp24 domain-containing protein 1) is a member of the TMED family of proteins (gene name TMED1). The TMED family of proteins are localized to membranes of the early secretory pathway, including the endoplasmic reticulum and Golgi, and function in vesicular protein trafficking (1, 2). TMED1 is a 59 kDa monomer and has been reported to exist as homodimer (3). TMED1 is composed of a 23 amino acid (aa) signal sequence, a 171 aa extra cellular domain, a 21 aa transmembrane domain, and a 12 aa cytoplasmic domain. The extracellular domain contains an 83 aa GOLD (Golgi Dynamics) domain, and COPI and COPII binding motifs are found in the cytoplasmic domain (1-3, 5). Human TMED1 shares 97% sequence identity with mouse, bovine, and rat homologs within the 171 aa extracellular domain. The beta -strand-rich GOLD domain has been specifically identified to be involved in intracellular protein trafficking (1, 4, 5). TMED1 is important in regulating innate immune signaling through its interaction with ST2L. Specifically, the GOLD domain in TMED1 interacts with the TIR domain of ST2L, a receptor for IL‑33 (1). This interaction promotes ST2L association with IL-33, allowing downstream signaling cascade activating MAP kinases, p38, and JNK (1, 6). Studies have shown knockdown of TMED-1 in HUVECs impairs the IL-33 induced response resulting in reduction of IL-6 and IL-8 productions (1).

References

Connolly, D. et al. (2013) J Biol Chem. 288:5616.
Gour, N. and Lajoie, S. (2018) Curr Allergy Ashma Rep. 16:65.
Jenne, N. (2002) J Biol Chem. 277:46504.
Anantharaman, V. and Aravind, L. (2002) Genome Biol. 3:research0023
Gomez-Navarro, N. and Miller, E. (2016) J Cell Biol. 215:769.
Hardman, C. and Ogg, G. (2016). Curr Opin Immunol. 42:16.

Entrez Gene IDs

11018 (Human); 17083 (Mouse); 315461 (Rat)

Alternate Names

IL1RL1-Binding Protein; Il1rl1l; IL1RL1LG; IL-1RL1LG; IL1RL1LGIL1RL1-binding protein; Interleukin 1 Receptor-Like 1 Ligand; Interleukin-1 receptor-like 1 ligand; Ly84l; MGC1270; P24g1; P24gamma1; Putative T1/ST2 receptor-binding protein; ST2L; T1/ST2 receptor binding protein; TMED1; Tp24; transmembrane emp24 domain containing 1; transmembrane emp24 domain-containing protein 1; transmembrane emp24 protein transport domain containing 1